Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-386

Background:

Description :

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyteendothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
Recombinant Human VCAM1 also called CD-106 is expressed in E. coli containing 674 (25-698) fused to an amino terminal hexahistidine tag.

Formulation:

Recombinant Human VCAM-1is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).

Application:

Recombinant VCAM1 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments.
The biological activity of this product has not yet been tested.

Storage Conditions:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please Avoid multiple freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:VCAM1
Synonyms:L1CAM

Protein synonyms/aliases:

Vascular cell adhesion protein 1 precursor (V-CAM 1)
    (CD106 antigen)
    (INCAM-100).

Protein Family:

Protein Domains:

Domains:
•IPR003987   Intercellular adhesion molecule/vascular cell adhesion molecule-1
•IPR007110   Immunoglobulin-like
•IPR003987   Intercellular adhesion molecule/vascular cell adhesion molecule-1
•IPR003987   Intercellular adhesion molecule/vascular cell adhesion molecule-1
•IPR008424   T-cell surface antigen CD2
•IPR013151   Immunoglobulin
•IPR007110   Immunoglobulin-like
•IPR007110   Immunoglobulin-like
•IPR008424   T-cell surface antigen CD2
•IPR013151   Immunoglobulin
•IPR007110   Immunoglobulin-like
•IPR007110   Immunoglobulin-like

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Difference in adhesion molecule expression (ICAM-1 and VCAM-1) in juvenile and adult dermatomyositis, polymyositis and inclusion body myositis.
Autoimmun Rev 2006 Feb;5(2):93-100
2. Soluble adhesion molecules ICAM-1, VCAM-1, P-selectin in children with Helicobacter pylori infection.
World J Gastroenterol 2005 Nov 21;11(43):6745-50
3. Nonhomogeneous density of CD34 and VCAM-1 alveolar capillaries in major types of idiopathic interstitial pneumonia.
Lung 2005 Sep-Oct;183(5):363-73
4. Expression of ICAM-1, ICAM-2, NCAM-1 and VCAM-1 by human synovial cells exposed to Borrelia burgdorferi in vitro.
Rheumatol Int 2005 Nov 24;:1-10
5. Transcriptional regulation of VCAM-1 expression by tumor necrosis factor-alpha in human tracheal smooth muscle cells: Involvement of MAPKs, NF-kappaB, p300, and histone acetylation.
J Cell Physiol 2006 Apr;207(1):174-86
6. Intermittent high glucose enhances ICAM-1, VCAM-1 and E-selectin expression in human umbilical vein endothelial cells in culture: the distinct role of protein kinase C and mitochondrial superoxide production.
Atherosclerosis 2005 Dec;183(2):259-67

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中華民國95年06月06日更新