Certificate of Analysis and Data Sheet

Source:
Escherichia Coli.

Catalog No.
ENZ-312

Background :

Description :

Urate oxidase catalyzes the enzymatic oxidation of uric acid into allantoin, an inactive and soluble metabolite. Recombinant Human Urate Oxidase produced in E.Coli is a tetrameric, non-glycosylated polypeptide chain containing 301 amino acids, having a molecular formula of C₁₅₂₃H₂₃₈₃N₄₁₇O₄₆₂S₇ and a molecular mass of 34,247 Dalton.

Formulation:

Each 1.5mg urate oxidase contains 5mg sucrose, 25mg glycine, 0.1mg Tween-80, 13.6mg Ma₂HPO₄*12H₂0 and 0.33mg NaH₂PO₄*2H₂0.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility:

It is recommended to reconstitute the lyophilized Recombinant UOX in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized rUOX although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Recombinant UOX should be stored at 4⁰C between 2-7 days and for future use below -18⁰C.
Please prevent freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing.
SDS-PAGE Silver Stained gel.

Biological Activity:

The specific activity was found to be 18U/mg.
One Unit oxidizes one micromole of uric acid per minute at 30蚓, at pH 8.9.

Amino acid sequence:

msavkaaryg kdnvrvykvh kdektgvqtv yemtvcvlle geietsytka dnsvivatds ikntiyitak qnpvtppelf gsilgthfie kynhihaahv nivchrwtrm didgkphphs firdseekrn vqvdvvegkg idiksslsgl tvlkstnsqf wgflrdeytt lketwdrils tdvdatwqwk nfsglqevrs hvpkfdatwa tarevtlktf aednsasvqa tmykmaeqil arqqlietve yslpnkhyfe idlswhkglq ntgknaevfa pqsdpnglik ctvgrsslks kl

Endotoxin:

Less than 10 ng/1.5mg of Urate Oxidase.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Procatalytic ligand strain. Ionization and perturbation of 8-nitroxanthine at the urate oxidase active site.
Biochemistry 2005 Aug 30;44(34):11440-6
2. Identification, cloning and expression of Pseudomonas aeruginosa Ps-x putative urate oxidase gene in Escherichia coli.
Pol J Microbiol 2004;53(4):227-36
3. Urate oxidase from Aspergillus flavus: new crystal-packing contacts in relation to the content of the active site.
Acta Crystallogr D Biol Crystallogr 2005 Mar;61(Pt 3):218-29
4. Urate oxidase (rasburicase) for treatment of severe tophaceous gout.
Nephrol Dial Transplant 2005 Feb;20(2):431-3
5. Recombinant urate oxidase (rasburicase) in the prophylaxis and treatment of tumor lysis syndrome.
Contrib Nephrol 2005;147:69-79
6. EPR spin trapping of a radical intermediate in the urate oxidase reaction.
Nucleosides Nucleotides Nucleic Acids 2004 Oct;23(8-9):1131-4