Certificate of Analysis and Data Sheet

Source:
Baculovirus Insect Cells.

Catalog No.
PRO-288

Background:

Thrombospondin-1 is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. Also, it has been studied for its use as a potential regulator of tumor growth and metastasis TSP expression is highly regulated by different hormones and cytokines and is developmentally controlled. TSP stimulates the growth of vascular smooth muscle cells and human foreskin fibroblasts,a combination of IFN-gamma and TNF-alpha inhibits TSP production in these cells.

Description :

Recombinant Human TSP is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. Recombinant TSP is Reactive with A4.1 anti-TSP mAb and its Mw is 14,000 dalton.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

The sterile protein solution (0.178mg/ml) contains 30mM Sodium phosphate, pH 7 and 500mM NaCl.

Stability:

Recombinant Human TSP should be stored between 2⁰C- 8⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human TSP-1.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:THBS1
Synonyms:TSP, TSP1

Protein synonyms/aliases:

Thrombospondin-1 precursor.

Protein Family:

Belongs to the thrombospondin family.

Protein Domains:

Domains:
•IPR001007   von Willebrand factor, type C
•IPR000884   Thrombospondin, type I
•IPR006209   EGF-like
•IPR013032   EGF-like region
•IPR006209   EGF-like
•IPR003367   Thrombospondin type 3 repeat
•IPR008859   Thrombospondin, C-terminal

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Impaired expression of thrombospondin-1 in eyes with age related macular degeneration.
Br J Ophthalmol 2006 Jan;90(1):48-54
2. Expression changes of thrombospondin-1 and neuropeptide Y in myocardium of STZ-induced rats.
Int J Cardiol 2005 Nov 2;105(2):192-7
3. Suppression of tumor cell invasion by cyclooxygenase inhibitors is mediated by thrombospondin-1 via the early growth response gene Egr-1.
Mol Cancer Ther 2005 Oct;4(10):1551-8
4. Oncogenes and Angiogenesis: down-regulation of thrombospondin-1 in normal fibroblasts exposed to factors from cancer cells harboring mutant ras.
Cancer Res 2005 Oct 1;65(19):8878-86
5. Strong expression of ID1 protein is associated with decreased survival, increased expression of ephrin-A1/EPHA2, and reduced thrombospondin-1 in malignant melanoma.
Br J Cancer 2005 Oct 17;93(8):933-8
6. Thrombospondin-1 is induced in rat myocardial infarction and its induction is accelerated by ischemia/reperfusion.
Exp Biol Med (Maywood) 2005 Oct;230(9):621-30

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中華民國95年06月06日更新