Source:
Sf9 insect cells.
Catalog No.
ENZ-306
Description : DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis.
In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kDa molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kDa molecular weight from which the Scl-70 antigen has derived its name.
Recombinant Human Scl-70 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 dalton.
Recombinant Scl-70 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered clear soultion.
Formulation: Recombinant Human Scl-70 is supplied at a of 0.9mg/ml in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol.
Stability: Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.
Immunological functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Coating concentration:
0.5-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Purity:
Greater than 80% (SDS-PAGE).
Usage: This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.
Gene:
Name:TOP1
DNA topoisomerase I (EC 5.99.1.2).
Belongs to the eukaryotic type I topoisomerase family.
Domains:
•IPR008336 Eukaryotic DNA topoisomerase I, DNA binding
•IPR001631 Eukaryotic-like DNA topoisomerase I
