Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-286

Background:

An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide.

Description :

Recombinant Human Cu/Zn Superoxide Dismutase produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 154 amino acids and having a total molecular mass of 31,608 Dalton.
The Cu/Zn SOD is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant Superoxide Dismutase was lyophilized after from a sterile solution containing 50mM sodium phosphate buffer and 100mM sodium chloride.

Solubility:

It is recommended to reconstitute the lyophilized SOD in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Superoxide Dismutase although stable at room temperature for 2 weeks, should be stored desiccated below -18 C. Upon reconstitution should be stored at 4 C. Please prevent freeze-thaw cycles.
Please prevent freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Lys-Ala-Val.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's recombinant Superoxide Dismutase is fully biologically active when compared to standard. The potency per mg was tested by Pyrogallic Acid method and was found to be 8232Units/mg.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of SOD.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of SOD as a Reference Standard.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:SOD1

Protein synonyms/aliases:

Superoxide dismutase [Cu-Zn (EC 1.15.1.1).

Protein Family:

Belongs to the Cu-Zn superoxide dismutase family.

Protein Domains:

Domains:
•IPR001424   Superoxide dismutase, copper/zinc binding

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Association of manganese superoxide dismutase expression with progression of carcinogenesis in Barrett esophagus.
Arch Surg 2005 Dec;140(12):1204-9; discussion 1209
2. Superoxide dismutase activity in colostrum, transitional and mature human milk.
Turk J Pediatr 2005 Oct-Dec;47(4):343-7
3. Relationship between eradication of Helicobacter pylori and gastric mucosal superoxide dismutase activity.
Anticancer Res 2005 Nov-Dec;25(6C):4763-7
4. Synergistic interaction between methotrexate and a superoxide dismutase mimetic: pharmacologic and potential clinical significance.
Arthritis Rheum 2005 Dec;52(12):3755-60
5. A rare Cu/Zn superoxide dismutase mutation causing familial amyotrophic lateral sclerosis with variable age of onset and incomplete penetrance in China.
Amyotroph Lateral Scler Other Motor Neuron Disord 2005 Dec;6(4):234-8
6. Characterisation of manganese superoxide dismutase from Phytophthora nicotianae.
Mycol Res 2005 Oct;109(Pt 10):1171-83

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中華民國95年06月06日更新