Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-221

Description :

The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541).
The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). Recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus.
Recombinant SHP-1 produced in E.Coli is a single,non-glycosylated polypeptide chain conjtaining 300 amino acids and having a molecular mass of 34.3 kDa.

Physical Appearance:

Sterile filtered clorless solution.

Formulation:

The protein (1mg/ml) contains 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mMDTT and 20%Glycerol.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks. Store, frozen at -20蚓 for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Sequence:

MGFWEEFES LQKQEVKNLH QRLEGQRPEN KGKNRYKNIL PFDHSRVILQ GRDSNIPGSDYINANYIKNQ LLGPDENAKT YIASQGCLEA TVNDFWQMAW QENSRVIVMT TREVEKGRNKCVPYWPEVGM QRAYGPYSVT NCGEHDTTEY KLRTLQVSPL DNGDLIREIW HYQYLSWPDHGVPSEPGGVL SFLDQINQRQ ESLPHAGPII VHCSAGIGRT GTIIVIDMLM ENISTKGLDCDIDIQKTIQM VRAQRSGMVQ TEAQYKFIYV AIAQFIETTK KKLEVLQSQK GQESEYGNITY

Activity:

5,000 U/mg

Unit Defenition:

One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37蚓 using 10mM of substrate.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PTPN6
Synonyms:HCP, PTP1C

Protein synonyms/aliases:

Tyrosine-protein phosphatase non-receptor type 6 (EC 3.1.3.48)
    (Protein-tyrosine phosphatase 1C)
    (PTP-1C)
    (Hematopoietic cellprotein-tyrosine phosphatase)
    (SH-PTP1)
    (Protein-tyrosine phosphataseSHP-1).

Protein Family:

Belongs to the protein-tyrosine phosphatase family.

Protein Domains:

Domains:
•IPR000980   SH2 motif
•IPR000242   Tyrosine specific protein phosphatase

Related proteins:

•Recombinant Human Protein Tyrosine Phosphatase 1B

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Decoding protein-protein interactions through combinatorial chemistry: sequence specificity of SHP-1, SHP-2, and SHIP SH2 domains.
Biochemistry 2005 Nov 15;44(45):14932-47
2. A SHPing tale: perspectives on the regulation of SHP-1 and SHP-2 tyrosine phosphatases by the C-terminal tail.
Cell Signal 2005 Nov;17(11):1323-32
3. Protein tyrosine phosphatase kappa and SHP-1 are involved in the regulation of cell-cell contacts at adherens junctions in the exocrine pancreas.
Gut 2005 Oct;54(10):1445-55
4. Deficiency of the Src homology region 2 domain-containing phosphatase 1 (SHP-1) causes enrichment of CD4+CD25+ regulatory T cells.
J Immunol 2005 Jun 1;174(11):6627-38
5. Regulation of the Leishmania-induced innate inflammatory response by the protein tyrosine phosphatase SHP-1.
Eur J Immunol 2005 Jun;35(6):1906-17
6. The tyrosine phosphatase, SHP-1, is a negative regulator of endothelial superoxide formation.
J Am Coll Cardiol 2005 May 17;45(10):1700-6

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中華民國95年06月06日更新