Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-325

Description :

Recombinant Human SDF-1b produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8508 Dalton.
Human SDF-1b is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

SDF-1b is lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized SDF-1beta in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized SDF-1beta although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution SDF-1b should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be
Lys-Pro-Val-Ser-Leu.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's SDF-1b is fully biologically active when compared to standard.
The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant Human Stromal Cell-Derived Factor-1 beta.

Protein content:

SDF-1b protein quantitation was carried out by two independent methods: 1. 1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Human Stromal Cell-Derived Factor-1 beta as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CXCL12
Synonyms:SDF1

Protein synonyms/aliases:

Stromal cell-derived factor 1 precursor (SDF-1)
    (CXCL12)
    (Pre-B cellgrowth-stimulating factor)
    (PBSF)
    (hIRH)

Precursor's functional components:

SDF-1-beta(3-72);SDF-1-alpha(3-67) .

Protein Family:

Belongs to the intercrine alpha (chemokine CxC)

Protein Domains:

Domains:
•IPR001811 Small chemokine, interleukin-8-like

Related proteins:

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. The SDF-1/CXCR4 pathway and the development of the cerebellar system.
Eur J Neurosci 2005 Oct;22(8):1831-9
2. Lack of association of SDF-1 3'A variant allele with long-term nonprogressive HIV-1 infection is extended beyond 16 years.
J Acquir Immune Defic Syndr 2005 Nov 1;40(3):276-9
3. The chemokine SDF-1/CXCL12 contributes to T lymphocyte recruitment in human pre-ovulatory follicles and coordinates with lymphocytes to increase granulosa cell survival and embryo quality.
Am J Reprod Immunol 2005 Nov;54(5):270-83
4. Haplotype analysis of the SDF-1 (CXCL12) gene in a longitudinal HIV-1/AIDS cohort study.
Genes Immun 2005 Dec;6(8):691-8
5. Chemokine receptor CXCR4-dependent internalization and resecretion of functional chemokine SDF-1 by bone marrow endothelial and stromal cells.
Nat Immunol 2005 Oct;6(10):1038-46
6. Complement C3a enhances CXCL12 (SDF-1)-mediated chemotaxis of bone marrow hematopoietic cells independently of C3a receptor.
J Immunol 2005 Sep 15;175(6):3698-706

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中華民國95年06月06日更新