Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-306

Background:

Although histochemical staining of the S100 protein family has been used for many years in the diagnosis of malignant melanoma, recent studies suggest one of the proteins comprising the S100 family, S100B, has particular utility in many aspects of the clinical management of malignant melanoma. This protein has been shown to be of use in staging malignant melanoma, in establishing prognosis, in evaluating treatment success and in predicting relapse. S100B is an independent prognostic factor and pretreatment circulating S100B concentrations predict duration of survival in melanoma patients. Survival is significantly longer in melanoma patients with normal S100B levels compared to those with elevated levels.

Description :

Recombinant S100b shows a band at 48kDa on SDS-PAGE commassie blue stained gel. Recombinant S100b is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered liquid.

Formulation:

0.55mg/ml in 50mM Bis-Tris-Propane, pH-7, 300mM NaCl and 0.020% NaN3.

Stability:

Store vial at -20C^o to -80C^o. When stored at the recommended temperature, recombinant human S100b is stable for 12 months.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates:

Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human S100b.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:S100B

Protein synonyms/aliases:

S-100 calcium-binding protein beta subunit (S-100 protein, betachain).

Protein Family:

Belongs to the S-100 family.

Protein Domains:

Domains:
•IPR002048   Calcium-binding EF-hand
•IPR001751   Calcium-binding protein, S-100/ICaBP type

Related proteins:

•Recombinant Rat Calbindin D-9K
•Recombinant Human S100P
•Recombinant Human S100Z
•Recombinant Human S100A1
•Recombinant Human S100A4
•Recombinant Human S100A10
•Recombinant Human S100A11

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Structure of the Ca(2+)/S100B/NDR Kinase Peptide Complex: Insights into S100 Target Specificity and Activation of the Kinase.
Biochemistry 2006 Feb 7;45(5):1536
2. S100B stimulates myoblast proliferation and inhibits myoblast differentiation by independently stimulating ERK1/2 and inhibiting p38 MAPK.
J Cell Physiol 2006 Jan 17;
3. S100B is a sensitive but not specific prognostic index in comatose patients after cardiac arrest.
Minerva Chir 2005 Dec;60(6):477-80
4. Serum and cereb rospinal fluid S100B concentrations in patients with neurocysticercosis.
Braz J Med Biol Res 2006 Jan;39(1):129-35
5. Systemic endotoxin administration results in increased S100B protein blood levels and periventricular brain white matter injury in the preterm fetal sheep.
Eur J Obstet Gynecol Reprod Biol 2006 Jan 1;124(1):15-22
6. S100B in Guillain-Barre syndrome.
Br J Anaesth 2006 Jan;96(1):141-2

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中華民國95年06月06日更新