Certificate of Analysis and Data Sheet
Source:
E.Coli
Catalog No.
PRO-385
Background:
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
Description :
The S100 family of proteins contain two EF-hand calcium-binding motifs and are thought to be involved in the regulation of a number of cellular processes including cell cycle progression and differentiation. This protein may function in motility, invasion and tubulin polymerisation.
Recombinant Human S100A11 also called S100C is expressed in E. coli having a molecular weight of 17kDa fused to an amino terminal hexahistidine tag.
Physical Appearance:
Sterile filtered liquid.
Formulation:
Recombinant Human S100-A11 is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).
Stability:
Store at 4蚓 if entire vial will be used within 1-2 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.
Purity:
Greater than 95% (SDS-PAGE).
2 bands on Western blot at 17 and 34 kDa, respectively representing monomeric and dimeric form.
Application:
Recombinant S100A11 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments.
The biological activity of this product has not yet been tested.
Usage:
This material is offered by ProSpec-TechnoGene for research,
laboratory or further evaluation purposes.
Gene:
Name:S100A11
Synonyms:MLN70, S100C
Protein synonyms/aliases:
Calgizzarin (S100 calcium-binding protein A11)
(S100C protein)
(MLN70).
Protein Family:
Belongs to the S-100 family.
Protein Domains:
Domains:
•IPR002048 Calcium-binding EF-hand
•IPR001751 Calcium-binding protein, S-100/ICaBP type
Related proteins:
•Recombinant Human S100b
•Recombinant Rat Calbindin D-9K
•Recombinant Human S100P
•Recombinant Human S100Z
•Recombinant Human S100A1
•Recombinant Human S100A4
•Recombinant Human S100A10
Protein links: