Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-364

Background:

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.

Description :

S100 belongs to the family of calcium binding proteins with EF-hand type Ca⁺² binding motive. S100A1 (Calcium Binding Protein A1) is involved in the activation of sarcoplasmatic calcium release and the regulation of intermediate filament polymerization.

Presentation:

100痞 purified human S100A4 protein at 0.1mg/ml in 20mM HEPES-KOH, pH7, 50mM NaCl, 1mM EDTA and 1mM DTT.

Application And Suggested Dilutions:

?ELISA ?Inhibition Assays ?Western Blotting

Characterization:

S100A1 antibody reactive.

Activity:

Assayed for calcium binding.

Purity:

Greater than 95% by SDS-PAGE.

Storage & Stability:

Store vial at -20⁰C to -80⁰C. When stored at the recommended temperature, this protein is stable for 12 months.

Legal Consideration:

This material is offered by ProSpec-TechnoGene for research, laboratory or further manufacturing purposes.

Gene:

Name:S100A1
Synonyms:S100A

Protein synonyms/aliases:

S-100 protein, alpha chain (S100 calcium-binding protein A1).

Protein Family:

Belongs to the S-100 family.

Protein Domains:

Domains:
•IPR002048   Calcium-binding EF-hand
•IPR001751   Calcium-binding protein, S-100/ICaBP type

Related proteins:

•Recombinant Human S100b
•Recombinant Human S100A4

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Co-expression/co-location of S100 proteins (S100B, S100A1 and S100A2) and protein kinase C (PKC-beta, -eta and -zeta) in a rat model of cerebral basilar artery vasospasm.
Neuropathol Appl Neurobiol 2005 Dec;31(6):649-60
2. S100A1 increases the gain of excitation-contraction coupling in isolated rabbit ventricular cardiomyocytes.
J Mol Cell Cardiol 2005 Dec;39(6):900-10
3. The three-dimensional solution structure of Ca(2+)-bound S100A1 as determined by NMR spectroscopy.
J Mol Biol 2005 Oct 21;353(2):410-26
4. S100A1 gene therapy preserves in vivo cardiac function after myocardial infarction.
Mol Ther 2005 Dec;12(6):1120-9
5. S100A1 enhances the L-type Ca2+ current in embryonic mouse and neonatal rat ventricular cardiomyocytes.
J Biol Chem 2005 Oct 28;280(43):36019-28
6. Affinity of S100A1 protein for calcium increases dramatically upon glutathionylation.
FEBS J 2005 May;272(10):2557-65

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中華民國95年06月06日更新