Source:
E.Coli
Catalog No.
CYT-456
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
Description: 9.9 kDa protein containing 93 amino acid residues.
MSSKTLCSME EAINERIQEV AGSLIFRAIS SIGLECQSVT SRGDLATCPR GFAVTGCTCG SACGSWDVRA ETTCHCQCAG MDWTGARCCR VQP
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Specificity: The amino acid sequence of the recombinant human Resistin is 100% homologous to the amino acid sequence of the human Resistin without signal sequence.
Purification Method: Two-step procedure using size exclusion chromatography before and after refolding.
Purity: >95% (SDS-PAGE analyzed). Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in 0.05 M phosphate buffer, 0.05 M NaCl, pH 7.2.
Solubility:
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Stability:
Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4蚓 for a limited period of time; it does not show any change after two weeks at 4蚓.
The lyophilized protein remains stable until the expiry date when stored at -20蚓.
Applications: Western blotting, ELISA
References: ?Steppan C.M., Lazar M.A.: Resistin and obesity-associated insulin resistance. TRENDS in Endocrinology and Metabolism. 13 (1), 18-23, (2002)
?Banerjee R.R., Lazar M.A.: Dimerization of resistin and resistin稺ike moleules is etermined by a single cystein. The Journal of Biological Chemistry. 276, 25970-25973, (2001)
?Kim KH. et al.: A Cysteine-rich Adipose Tissue-specific Secretory Factors Inhibits Adipocte Differentiation. The Journal of Biological Chemistry. 276 (14), 11252-11256, (2001)
?Steppan C.M. et al.: The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001)
?Way J.M. et al.: Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor g Agonists. The Journal of Biological Chemistry. 276 (28), 25651-25653, (2001).
?Hartman H.B., Lazar M.A. at al.: Mechanisms Regulating Adipocyte Expression of Resistin. The Journal of Biological Chemistry. 277 (22), 19754-19761, (2002)
?Fasshauer M. at al.: Tumor Necrosis Factor a Is a Negative Regulator of Resistin Gene Expression and Secretion in 3T3-L1 Adipocytes. Biochemical and Biophysical Research Communications. 208, 1027-1031, (2001).
?Steppan C.M. at al.: A Family of Tissue-specific Resistin-like Molecules. Proc. Natl. Acad. Sci.USA. 98 (2), 502-506, (2001)
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:RETN
Synonyms:FIZZ3, HXCP1, RSTN
ORFNames:UNQ407/PRO1199
Resistin precursor (Cysteine-rich secreted protein FIZZ3)
(Adiposetissue-specific secretory factor)
(ADSF)
(C/EBP-epsilon regulatedmyeloid-specific secreted cysteine-rich protein)
(Cysteine-richsecreted protein A12-alpha-like 2).
Belongs to the resistin/FIZZ family.
Domains:
•IPR009714 Resistin
•Recombinant Mouse Resistin like alpha (RELMalpha)
•Recombinant Human Resistin like beta (RELMbeta/FIZZ2)
•Recombinant Mouse Resistin (FIZZ3)
•Recombinant Mouse Resistin Like gamma
•Recombinant Rat Resistin
