Certificate of Analysis and Data Sheet

Source:
Escherichia Coli

Catalog No.
PRO-437

Background:

The retinoid X receptor(RXR) is a pleiotropic nuclear receptor transcription factor that interacts with a variety of nuclear receptor dimeric partner. RXR binds cognate response elements as a homodimer in the presence of its ligand, 9-cis retinoic acid, or as a heterodimer with other members of the nuclear hormone receptor superfamily including retinoic acid receptors(RAR), thyroid hormone receptors(TR), vitamin D receptors and peroxisome proliferators-activated receptors(PPAR). The RXR family includes three difference isoforms ; RXR alpha, beta, gamma. Human RXR a gene is localized on 9q34.9 and encodes two major isoforms(RXR a1, RXR a2).

Description:

Recombinant Human RXR-a produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids & having a molecular mass of 13.6 kDa.
The DNA binding domain of RXR(111-228aa) was purified by using conventional column chromatography techniques.

Physical Appearance:

Sterile filtered clorless solution.

Formulation:

The protein (1mg/ml) containing 20mM Tris-HCl pH7.5, 0.1M NaCl, 5mM beta-Mercaptoethanol.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Sequence:

MLGLNGVLKV PAHPSGNMAS FTKHICAICG DRSSGKHYGV YSCEGCKGFF KRTVRKDLTY TCRDNKDCLI DKRQRNRCQY CRYQKCLAMG MKREAVQEER QRGKDRNENE VESTSSANE

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:RXRA
Synonyms:NR2B1

Protein synonyms/aliases:

Retinoic acid receptor RXR-alpha (Retinoid X receptor alpha).

Protein Family:

Belongs to the nuclear hormone receptor family. NR2

Protein Domains:

Domains:
•IPR001628   Nuclear hormone receptor, DNA-binding
•IPR000536   Nuclear hormone receptor, ligand-binding

Related proteins:

•Recombinant Human Peroxisome Proliferator Activated Receptor Gamma

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Epicardial retinoid X receptor alpha is required for myocardial growth and coronary artery formation.
Proc Natl Acad Sci U S A 2005 Dec 20;102(51):18455-60
2. Retinoid X receptor alpha Regulates the expression of glutathione s-transferase genes and modulates acetaminophen-glutathione conjugation in mouse liver.
Mol Pharmacol 2005 Dec;68(6):1590-6
3. Retinoid X receptor-alpha-dependent transactivation by a naturally occurring structural variant of human constitutive androstane receptor (NR1I3).
Mol Pharmacol 2005 Nov;68(5):1239-53
4. Electrospray mass spectrometry for the direct accurate mass measurement of ligands in complex with the retinoid X receptor alpha ligand binding domain.
J Am Soc Mass Spectrom 2005 Oct;16(10):1631-40
5. Retinoic acid administration and vitamin A status modulate retinoid X receptor alpha and retinoic acid receptor alpha levels in mouse brown adipose tissue.
Mol Cell Biochem 2004 Nov;266(1-2):25-30
6. Retinoid X receptor alpha (RXRalpha) helix 12 plays an inhibitory role in the recruitment of the p160 co-activators by unliganded RXRalpha/retinoic acid receptor alpha heterodimers.
J Biol Chem 2004 Oct 22;279(43):45208-18

採購找生工，預算最輕鬆。

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中華民國95年06月06日更新