Source:
E.Coli
Catalog No.
CYT-454
RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant.
The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.
Description: Recombinant Human RELM-beta is a His-Tagged Fusion Protein, 11 kDa protein containing 90 amino acid residues of the human RELM-beta and 12 additional amino acid residues - HisTag (underlined).
Description: MG STQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQLETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Specificity: The amino acid sequence of the recombinant human RELM-beta is 100% homologous to the amino acid sequence of the human RELM-beta without signal sequence.
Purification Method: Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Purity: >95% (SDS-PAGE analyzed). Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH4.
Solubility:
Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.
Stability:
Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4蚓 for a limited period of time; it does not show any change after two weeks at 4蚓.
The lyophilized protein remains stable until the expiry date when stored at -20蚓.
Applications: Western blotting, ELISA
References: ?Banerjee RR, Lazar MA. Dimerization of resistin and resistin-like molecules is determined by a single cysteine. J Biol Chem. 2001 Jul 13; 276(28): 25970-3. Epub 2001 May 17.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:RETNLB
Synonyms:CCRG, FIZZ2, HXCP2, RETNL2
ORFNames:UNQ408/PRO770
Resistin-like beta precursor (RELMbeta)
(Cysteine-rich secretedprotein FIZZ2)
(Colon and small intestine-specific cysteine-richprotein)
(Cysteine-rich secreted protein A12-alpha-like 1)
(Coloncarcinoma-related gene protein).
Belongs to the resistin/FIZZ family.
Domains:
•IPR009714 Resistin
•Recombinant Mouse Resistin like alpha (RELMalpha)
•Recombinant Human Resistin (FIZZ3)
•Recombinant Mouse Resistin (FIZZ3)
•Recombinant Mouse Resistin Like gamma
•Recombinant Rat Resistin
