Certificate of Analysis and Data Sheet

Source:
E.coli

Catalog No.
HOR-245

Background:

Prouroguanylin is a 112-amino-acid prohormone precursor of uroguanylin- mature biologically active 16-amino-acid peptide cleaved from its C-terminus. Guanylin binds to receptor-guanylate cyclases (CG-C) resulting in increased intracellular cGMP levels leading to CFTCR (Cystic Fibrosis Transmembrane Conductance Regulator) activation and subsequent rise in fluid and electrolyte secretion into the lumen. Prouroguanyline knock-out mice showed impaired renal excretion of external NaCl leasing to elevated blood pressure independent of the level of diatary salt intake. Prouroguanylin is the predominant circulating molecular form found in circulation and in biological fluids.

Description:

The Recombinant Human Prouroguanylin is 10 kDa protein containing 88 amino acid residues of the human prouroguanylin and 2 additional amino acid residues.

Amino Acid sequence:

GPVYIQYQGF RVQLESMKKL SDLEAQWAPS PRLQAQSLLP AVCHHPALPQ DLQPVCASQE ASSIFKTLRT IANDDCELCV NVACTGCL

Physical Appearance:

Sterile Filtered lyophilized (freeze-dried) powder.

Formulation:

Sterile filtered and lyophilized from 0.5 mg/ml in 50mM Phosphate buffer pH7.4.

Specificity:

The recombinant sequnce is 100% homologous to the native human sequence.

Purification Method:

Three-step procedure using IMAC and size exclusion chromatography before and after refolding.

Purity:

>95% (SDS-PAGE and HPLC analyzed).

Reconstitution:

Add 0.2 ml of deionized H₂O and let the lyophilized pellet dissolve completely.

Stability/Shelf Life:

Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid repeated thaw-freeze cycles and store at -80蚓. Reconstituted protein can be stored at 4蚓 for a limited period of time.
The lyophilized protein remains stable until the expiry date when stored at -20蚓.

Applications:

Western blotting, ELISA

References:

?Cui L, Blanchard RK, Coy LM, Cousins RJ. Prouroguanylin overproduction and localization in the intestine of zinc-deficient rats. J Nutr. 2000 Nov;130(11):2726-32.
?Miyazato M, Nakazato M, Matsukura S, Kangawa K, Matsuo H. Genomic structure and chromosomal localization of human uroguanylin. Genomics. 1997 Aug 1;43(3):359-65.
?Hamra FK, Fan X, Krause WJ, Freeman RH, Chin DT, Smith CE, Currie MG, Forte LR. Prouroguanylin and proguanylin: purification from colon, structure, and modulation of bioactivity by proteases. Endocrinology. 1996 Jan;137(1):257-65.
?Miyazato M, Nakazato M, Yamaguchi H, Date Y, Kojima M, Kangawa K, Matsuo H, Matsukura S. Cloning and characterization of a cDNA encoding a precursor for human uroguanylin. Biochem Biophys Res Commun. 1996 Feb 15;219(2):644-8.
?Nakazato M, Yamaguchi H, Kinoshita H, Kangawa K, Matsuo H, Chino N, Matsukura S. Identification of biologically active and inactive human uroguanylins in plasma and urine and the increases in renal insufficiency. Biochem Biophys Res Commun. 1996 Mar 27;220(3):586-93.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:GUCA2B

Protein synonyms/aliases:

Guanylate cyclase activator 2B precursor

Precursor's functional components:

Guanylate cyclaseC-activating peptide 2 (Guanylate cyclase C-activating peptide II)
    (GCAP-II)
Uroguanylin (UGN) .

Protein Family:

Belongs to the guanylin family.

Protein Domains:

Domains:
•IPR000879   Guanylin

Related proteins:

•Recombinant Human Proguanylin

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新