Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CYT-267

Background:

Prolactin is a lactogenic hormone secreted by the adenohypophysis .Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation.Prolactin has been shown also to have cytokine-like activities and to have important immunoregulatory activities. It contributes to the development of lymphoid tissues and the maintenance of physiological immune function and also modulates a variety of T-cell immune responses. Prolactin has been reported to activate cellular proliferation in nonreproductive tissue, such as liver, spleen, and thymus. It induces significant proliferation in aortic smooth muscle cells and also enhances proliferation of these cells induced by PDGF . Prolactin also appears to be directly mitogenic for pancreatic beta cells. Prolactin is also mitogenic for cultured astrocytes.

Description :

Recombinant Human Prolactin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23007 Dalton.
Recombinant Human Prolactin is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant Prolactin was lyophilized from a concentrated (1mg/ml) solution with 10mM sodium Phosphate buffer pH=8 and 50mMNacl.

Solubility:

It is recommended to reconstitute the lyophilized Prolactin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Prolactin should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ile-Cys.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's Prolactin is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be < 0.065 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Prolactin.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:PRL

Protein synonyms/aliases:

Prolactin precursor (PRL).

Protein Family:

Belongs to the somatotropin/prolactin family.

Protein Domains:

Domains:
•IPR001400   Somatotropin hormone

Related proteins:

•Recombinant Ovine Prolactin
•Recombinant Human Placental-Corr Growth Hormone-22K
•Recombinant Human Pituitary Growth Hormone-20K
•Recombinant Rat Growth Hormone
•Recombinant Ovine Growth Hormone
•Recombinant bream Growth Hormone

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. The role of human prolactin and its antagonist, G129R, in mammary gland development and DMBA-initiated tumorigenesis in transgenic mice.
Int J Oncol 2005 Nov;27(5):1381-9
2. Solution structure of human prolactin.
J Mol Biol 2005 Aug 26;351(4):810-23
3. The antiangiogenic factor, 16-kDa human prolactin, induces endothelial cell cycle arrest by acting at both the G0-G1 and the G2-M phases.
Mol Endocrinol 2005 Jul;19(7):1932-42
4. Antitumor effects of recombinant human prolactin in human adenocarcinoma-bearing SCID mice with human NK cell xenograft.
Int Immunopharmacol 2005 Feb;5(2):417-25
5. EGF stimulates Pit-1 independent transcription of the human prolactin pituitary promoter in human breast cancer SK-BR-3 cells through its proximal AP-1 response element.
Mol Cell Endocrinol 2005 Jan 14;229(1-2):127-39
6. Mechanism for ordered receptor binding by human prolactin.
Biochemistry 2004 Nov 2;43(43):13755-65

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中華民國95年06月06日更新