Source:
E.Coli
Catalog No.
HOR-247
Formulation: The protein (1mg/ml) was lyophilized after extensive dialyses against 1.15mg sodium citrate, sodium chloride 7.31mg, 0.21mg citric acid, 0.1117 EDTA-Na2, 0.2mg Tween 80 and 50mgs Mannitol. Solubility: It is recommended to reconstitute the lyophilized rHuPTH in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized rHuPTH although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuPTH should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. Amino-Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Val-Ser-Glu-Ile.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's rHuPTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 1.0 x 104 Units/mg.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of rHuPTH.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of PTH as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:PTH
Parathyroid hormone precursor (Parathyrin)
(PTH)
(Parathormone).
Belongs to the parathyroid hormone family.
Domains:
•IPR001415 Parathyroid hormone
