Certificate of Analysis and Data Sheet

Source:
E.coli

Catalog No.
PRO-305

Background:

Description :

PTEN, a tumor suppressor, has been implicated in a large number of human tumors and is conserved from humans to worms. Characterization of PTEN protein showed that it is a phosphatase that acts on proteins and on 3-phosphorylated phosphoinositides, and can therefore modulate signaltransduction pathways that involve lipid second messengers. Recent results indicate that at least part of its role is to regulate the activity of the serine/threonine kinase AKT/PKB, and thus influence cell survival signaling.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

purified human PTEN protein at 0.1mg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% glycerol.

Stability:

Store vial at -20⁰C to -80⁰C. When stored at the recommended temperature, this protein is stable for 12 months.
Please avoid freeze-thaw cycles.

Application And Suggested Dilution:

?ELISA
?Inhibition Assays
?Western Blotting

Characterization:

On SDS-PAGE commassie blue stained gel, the purified recombinant PTEN shows a band at 86kDa antibody reactive.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PTEN
Synonyms:MMAC1, TEP1

Protein synonyms/aliases:

Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase PTEN(EC 3.1.3.67)
    (Mutated in multiple advanced cancers 1).

Protein Family:

Protein Domains:

Domains:
•IPR000340   Dual specificity protein phosphatase

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. [The expression of tumor suppressor gene PTEN protein and focal adhesion kinase in salivary adenoid cystic carcinoma and its significance.]
Shanghai Kou Qiang Yi Xue 2005 Dec;14(6):578-81
2. Differential PTEN protein expression profiles in superficial versus invasive bladder cancers.
Urol Int 2005;75(2):102-6
3. Reduced expression of PTEN protein and its prognostic implications in invasive ductal carcinoma of the breast.
Oncology 2005;68(4-6):398-404
4. Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases.
J Biol Chem 2005 Aug 12;280(32):28936-43
5. Functional definition of relevant epitopes on the tumor suppressor PTEN protein.
Cancer Lett 2005 Jun 8;223(2):303-12
6. Reduction of PTEN protein and loss of epidermal growth factor rec eptor gene mutation in lung cancer with natural resistance to gefitinib (IRESSA).
Br J Cancer 2005 May 9;92(9):1711-9

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中華民國95年06月06日更新