Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-224

Background :

Human Phosphoserine phosphatase (hPSP) is an important enzyme in the phosphorylated pathway of serine biosynthesis, which contributes a major portion of the endogenous L-serine. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2+-dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. Recently, its complex structures reveal that the open-closed environmental change of the active site, generated -helical bundle domain, is important to substrate by local rearrangement of the recognition and hydrolysis.

Description :

Recombinant Phosphoserine Phosphatase produced in E.Coli is a single,non-glycosylated polypeptide chain conjtaining 225 amino acids and having a molecular mass of 25 kDa.
Recombinant human PSP was overexpressed in E. coli and purified by conventional chromatography.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

The protein (1mg/ml) contains 20mM Hepes pH 7.5, 1mM DTT &100mM KCl2.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks. Store, frozen at -20蚓 for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Sequence:

MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEE.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PSPH

Protein synonyms/aliases:

Phosphoserine phosphatase (EC 3.1.3.3)
    (PSP)
    (O-phosphoserinephosphohydrolase)
    (PSPase)
    (L-3-phosphoserine phosphatase).

Protein Family:

Belongs to the serB family.

Protein Domains:

Domains:
•IPR005834   Haloacid dehalogenase-like hydrolase

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. How calcium inhibits the magnesium-dependent enzyme human phosphoserine phosphatase.
Eur J Biochem 2004 Aug;271(16):3421-7
2. A plasmid-encoded class 1 integron carrying sat, a putative phosphoserine phosphatase gene and aadA2 from enterotoxigenic Escherichia coli O159 isolated in Japan.
FEMS Microbiol Lett 2004 Jun 15;235(2):243-8
3. High-resolution structure of human phosphoserine phosphatase in open conformation.
Acta Crystallogr D Biol Crystallogr 2003 Jun;59(Pt 6):971-7
4. Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase.
J Biol Chem 2002 Nov 29;277(48):46651-8
5. Structural characterization of the reaction pathway in phosphoserine phosphatase: crystallographic " snapshots " of intermediate states.
J Mol Biol 2002 May 31;319(2):421-31
6. Purification, crystallization and preliminary X-ray diffraction analysis of human phosphoserine phosphatase.
Acta Crystallogr D Biol Crystallogr 2002 Jan;58(Pt 1):133-4

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中華民國95年06月06日更新