Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ENZ-331

Background:

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

Description :

Recombinant Human PPIase produced in E.Coli is a single,non-glycosylated polypeptide chain containing 163 amino acids & having a molecular mass of 18.2 kDa.

Physical Appearance:

Sterile filtered clorless solution.

Formulation:

The protein (1mg/ml) containing 20mM Tris-HCl buffer (pH7.5) 0.1M NaCl, 5mM DTT & 20% Glycerol.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Sequence:

MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PIN1

Protein synonyms/aliases:

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (EC 5.2.1.8)
    (Rotamase Pin1)
    (PPIase Pin1).

Protein Family:

Protein Domains:

Domains:
•IPR001202   WW/Rsp5/WWP
•IPR000297   PpiC-type peptidyl-prolyl cis-trans isomerase

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Activation of beta-catenin signaling in prostate cancer by peptidyl-prolyl isomerase Pin1-mediated abrogation of the androgen receptor-beta-catenin interaction.
Mol Cell Biol 2006 Feb;26(3):929-39
2. Expression of Pin1, A Peptidyl-Prolyl Isomerase, in the Ovaries of eCG/hCG-treated Immature Female Mice.
J Reprod Dev 2005 Dec 28;
3. PIN1 promoter polymorphisms are associated with Alzheimer's disease.
Neurobiol Aging 2005 Dec 26;
4. Pin1: a proline isomerase that makes you wheeze?
Nat Immunol 2005 Dec;6(12):1211-2
5. The prolyl-isomerase Pin1 accumulates in the Lewy bodies of Parkinson's disease and facilitates the formation of alpha -synuclein inclusions.
J Biol Chem 2005 Dec 19;
6. The peptidyl-prolyl isomerase Pin1 regulates the stability of granulocyte-macrophage colony-stimulating factor mRNA in activated eosinophils.
Nat Immunol 2005 Dec;6(12):1280-7

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新