Certificate of Analysis and Data Sheet
Source:
E.Coli
Catalog No.
PKA-228
Background :
The peroxisome proliferator activated receptors (PPARs) are ligandactivated transcription factors within the nuclear receptor superfamily, which play important roles in adipogenesis, glucose homeostasis and inflammation. Three different isotypes can be distinguished; alpha, beta and gamma. PPAR-g, is mainly expressed in adipose tissue and to lesser extent in colon, the immune system and the retina. Whereas PPARa operates in the catabolism of fatty acids in the liver, PPAR gamma influences the storage of fatty acids in the adipose tissue and plays a role in adipocyte differentiation. PPARg binds to DNA as a heterodimer with retinoid X receptor (RXR), activating expression of target genes after binding ligand. Recombinant Human Peroxisome Proliferator Activated Receptor Gamma is expressed in E. coli having a molecular weight of 59.2 kDa and fused to an amino terminal hexahistidine tag.
Description :
Recombinant Human PPAR-g is the full-length form of the protein fused with GST and carries a histidine tag on the N-terminus.
Physical Appearance:
Sterile Filtered clear soultion.
Formulation:
1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).
Storage:
Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.
Purity:
Greater than 95% (SDS-PAGE).
Mass spectrometry of tryptic digest products for identity determination.
Usage:
This material is offered by ProSpec-TechnoGene for research,
laboratory or further evaluation purposes.
Gene:
Name:PPARG
Synonyms:NR1C3
Protein synonyms/aliases:
Peroxisome proliferator-activated receptor gamma (PPAR-gamma).
Protein Family:
Belongs to the nuclear hormone receptor family. NR1
Protein Domains:
Domains:
•IPR001628 Nuclear hormone receptor, DNA-binding
•IPR000536 Nuclear hormone receptor, ligand-binding
Related proteins:
• Recombinant Human Retinoid X Receptor Alpha
Protein links:
