Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ENZ-300

Background :

Paraoxonase 2 (PON2) is a member of a multigene family whose genes share 65% identity at the amino acid level, and is expressed in a variety of tissues, including the pancreas. PON2 overexpression has been shown to lower the intracellular oxidative state and reduce the cells ability to oxidize LDL. PON2 is therefore implicated in the modulation of oxidative stress.

Description :

Recombinant Human Paraoxonase-2 is expressed in E. coli having a molecular weight of 43.5 kDa and fused to an amino terminal hexahistidine tag.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human PON2 is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).

Stability:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Applications:

rHuPON-2 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments.
The biological activity of this product has not yet been tested.

Purity:

Greater than 95% (SDS-PAGE). Single band on Western Blot

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PON2

Protein synonyms/aliases:

Serum paraoxonase/arylesterase 2 (EC 3.1.1.2)
    (EC 3.1.8.1)
    (PON 2)
    (Serum aryldialkylphosphatase 2)
    (A-esterase 2)
    (Aromatic esterase 2).

Protein Family:

Belongs to the paraoxonase family.

Protein Domains:

Domains:
•IPR002640   Arylesterase

Related proteins:

•Recombinant Human Paraoxonase-1

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. The paraoxonase-2-310 polymorphism is associated with the presence of microvascular complications in diabetes mellitus.
J Intern Med 2005 Oct;258(4):363-8
2. [Genetic polymorphism of pyruvate dehydrogenase kinase 4 (PDK4), paraoxonase 2 (PON2), and fatty acid binding protein 2 (FABP2) in the NIDDM population of Senegal]
Dakar Med 2002;47(1):18-21
3. Paraoxonase 2 (PON2) expression is upregulated via a reduced-nicotinamide-adenine-dinucleotide-phosphate (NADPH)-oxidase-dependent mechanism during monocytes differentiation into macrophages.
Free Radic Biol Med 2004 Dec 15;37(12):2052-63
4. Possible association between Cys311Ser polymorphism of paraoxonase 2 gene and late-onset Alzheimer's disease in Chinese.
Brain Res Mol Brain Res 2004 Jan 5;120(2):201-4
5. Decreased macrophage paraoxonase 2 expression in patients with hypercholesterolemia is the result of their increased cellular cholesterol content: effect of atorvastatin therapy.
Arterioscler Thromb Vasc Biol 2004 Jan;24(1):175-80
6. [The association of paraoxonase 2 gene C311S variant with ischemic stroke in Chinese type 2 diabetes mellitus patients]
Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2003 Jun;20(3):215-9

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新