Certificate of Analysis and Data Sheet

Source:
Insect Cells

Catalog No.
CYT-420

Background:

Placenta growth factor (PlGF), a homodimeric glycoprotein that is homologous to vascular endothelial growth factor (VEGF), is mitogenic to endothelial cells and protects trophoblast from apoptosis. Alternative splicing of mature mRNA gives rise to three known isoforms of PlGF. PlGF is expressed by human trophoblast during normal pregnancy, however, it is not known which isoforms are produced.

Description:

Recombinant Human PLGF-2 produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 152 amino acids and having a total molecular mass of 44 kDa. Recombinant Human PLGF-2 is purified by proprietary chromatographic techniques

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a concentrated (1mg/ml) solution containing BSA.

Solubility:

It is recommended to reconstitute the lyophilized Recombinant PLGF-2 in sterile 20mM acetic acid not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Human PLGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Human PLGF-2 should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 80.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec旧 PLGF-2 is fully biologically active when compared to standard.
Recombinant human PlGF-2 can bind to immobilized rh-sFlt-1 (100ng/well) with a linear range at 0.3 ?10ng/mL.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant PLGF-2.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of PLGF-2 as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:PGF
Synonyms:PLGF

Protein synonyms/aliases:

Placenta growth factor precursor (PlGF).

Protein Family:

Belongs to the PDGF/VEGF growth factor family.

Protein Domains:

Domains:
•IPR000072   Platelet-derived growth factor (PDGF)

Related proteins:

•Recombinant Human Platelet Derived Growth Factor-AA
•Recombinant Human Platelet Derived Growth Factor-BB
•Recombinant Mouse Platelet Derived Growth Factor-BB
•Recombinant Human Placenta Growth Factor-1
•Recombinant Bovine Vacular Endothelial Growth Factor
•Recombinant Human Vacular Endothelial Growth Factor
•Recombinant Mouse Vacular Endothelial Growth Factor
•Recombinant Rat Vacular Endothelial Growth Factor

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

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中華民國95年06月06日更新