Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells.

Catalog No.
ENZ-304

Background:

Threonyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases.

Description :

Recombinant Human PL-7 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 83,312 dalton.
Recombinant PL-7 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human PL-7 is supplied at a of 0.9mg/ml in 16mM HEPES buffer pH-8, 200mM sodium chloride, and 20% glycerol.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% (SDS-PAGE).

Immunological functions:

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).

Applications:

Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.

Coating concentration:

0.3-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further manufacturing purposes.

Gene:

Name:TARS

Protein synonyms/aliases:

Threonyl-tRNA synthetase, cytoplasmic (EC 6.1.1.3)
    (Threonine--tRNAligase)
    (ThrRS).

Protein Family:

Belongs to the class-II aminoacyl-tRNA synthetase

Protein Domains:

Domains:
•IPR004095   TGS
•IPR012947   Threonyl/alanyl tRNA synthetase, SAD
•IPR002314   tRNA synthetase, class II (G, H, P and S)
•IPR004154   Anticodon-binding

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Achieving error-free translation; the mechanism of proofreading of threonyl-tRNA synthetase at atomic resolution.
Mol Cell 2004 Nov 5;16(3):375-86
2. Induced fit and kinetic mechanism of adenylation catalyzed by Escherichia coli threonyl-tRNA synthetase.
Biochemistry 2003 Dec 30;42(51):15102-13
3. Anti-angiogenesis effects of borrelidin are mediated through distinct pathways: threonyl-tRNA synthetase and caspases are independently involved in suppression of proliferation and induction of apoptosis in endothelial cells.
J Antibiot (Tokyo) 2003 Aug;56(8):709-15
4. Threonyl-tRNA synthetase of archaea: importance of the discriminator base in the aminoacylation of threonine tRNA.
Nucleic Acids Symp Ser 2000;(44):83-4
5. Molecular recognition of threonine tRNA by threonyl-tRNA synthetase from an extreme thermophilic archaeon, Aeropyrum pernix K1.
Nucleic Acids Res Suppl 2002;(2):81-2
6. Conformational movements and cooperativity upon amino acid, ATP and tRNA binding in threonyl-tRNA synthetase.
J Mol Biol 2003 Aug 1;331(1):201-11

採購找生工，預算最輕鬆。

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中華民國95年06月06日更新