Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells.

Catalog No.
ENZ-305

Background:

Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.

Description :

Recombinant Human PL-12 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 dalton.
Recombinant PL-12 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human PL-12 is supplied at a of 0.9mg/ml in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% Glycerol.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% (SDS-PAGE).

Immunological functions:

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).

Applications:

Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.

Coating concentration:

0.3-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further manufacturing purposes.

Gene:

Name:AARS

Protein synonyms/aliases:

Alanyl-tRNA synthetase (EC 6.1.1.7)
    (Alanine--tRNA ligase)
    (AlaRS).

Protein Family:

Belongs to the class-II aminoacyl-tRNA synthetase

Protein Domains:

Domains:
•IPR012947   Threonyl/alanyl tRNA synthetase, SAD
•IPR003156   Phosphoesterase, DHHA1
•IPR002318   Alanyl-tRNA synthetase, class IIc

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Long-term clinical course of a patient with anti PL-12 antibody accompanied by interstitial pneumonia and severe pulmonary hypertension.
Intern Med 2005 Apr;44(4):319-25
2. Clinical manifestations in patients with antibody to PL-12 antigen (alanyl-tRNA synthetase).
Am J Med 1990 Mar;88(3):241-51

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中華民國95年06月06日更新