Certificate of Analysis and Data Sheet

Source:
SF9 insect cells.

Catalog No.
PKA-324

Description :

Recombinant Human PKC-teta produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-706 and having a molecular mass of 86 KD. PKC-t is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human PKC-teta is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.

Unit Definition:

~ 1800 units/mg. One unit of PKCt activity transfers 1 nmole of phosphate to a PKC-t pseudosubstrate peptide per minute at 30⁰C using. Recombinant human active PKC-teta also phosphorylates histone, MARCKS, moesin, RasGRP3, and PKC alpha and PKC epsilon pseudosubstrates. PKC-teta is a novel PKC and does not require calcium for activity. Activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. PKC-teta is involved in T-cell activation and T-cell receptor signaling including T-cell mediated activation of NF-kB and AP-1.

Storage:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 85% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PRKCQ
Synonyms:PRKCT

Protein synonyms/aliases:

Protein kinase C theta type (EC 2.7.1.-)
    (nPKC-theta).

Protein Family:

Belongs to the Ser/Thr protein kinase family. PKC

Protein Domains:

Domains:
•IPR002219   Protein kinase C, phorbol ester/diacylglycerol binding
•IPR000719   Protein kinase
•IPR000961   Protein kinase, C-terminal

Related proteins:

•Recombinant Human Protein Kinase C alpha
•Recombinant Human Protein Kinase C beta II
•Recombinant Human Protein Kinase C beta I
•Recombinant Human Protein Kinase C gamma
•Recombinant Human Protein Kinase C delta
•Recombinant Human Protein Kinase C e

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Menin represses JunD transcriptional activity in protein kinase C theta-mediated Nur77 expression.
Exp Mol Med 2005 Oct 31;37(5):466-75
2. Protein kinase C-theta is an early survival factor required for differentiation of effector CD8+ T cells.
J Immunol 2005 Oct 15;175(8):5126-34
3. Characterization of protein kinase C theta activation loop autophosphorylation and the kinase domain catalytic mechanism.
Biochemistry 2005 Jul 19;44(28):9563-73
4. Flavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells.
Br J Pharmacol 2005 Aug;145(7):934-44
5. Protein kinase C theta is not essential for T-cell-mediated clearance of murine gammahe rpesvirus 68.
J Virol 2005 Jun;79(11):6808-13
6. Clustering of T cell ligands on artificial APC membranes influences T cell activation and protein kinase C theta translocation to the T cell plasma membrane.
J Immunol 2005 Mar 15;174(6):3204-11

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中華民國95年06月06日更新