Certificate of Analysis and Data Sheet

Source:
SF9 insect cells.

Catalog No.
PKA-320

Description :

Recombinant Human PKC-gamma produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-676 and having a molecular mass of 80 KD. PKC-g is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human PKC-g is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.

Unit Definition:

6000-8000 units/mg. One unit of PKC-gamma activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30蚓. Recombinant human active PKC-g also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3beta. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.

Storage:

Store at 4蚓 if entire vial will be used within 2-4 weeks. Store frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PRKCG
Synonyms:PKCG

Protein synonyms/aliases:

Protein kinase C gamma type (EC 2.7.1.37)
    (PKC-gamma).

Protein Family:

Belongs to the Ser/Thr protein kinase family. PKC

Protein Domains:

Domains:
•IPR002219   Protein kinase C, phorbol ester/diacylglycerol binding
•IPR000008   C2
•IPR000719   Protein kinase
•IPR000961   Protein kinase, C-terminal

Related proteins:

•Recombinant Human Protein Kinase C alpha
•Recombinant Human Protein Kinase C beta II
•Recombinant Human Protein Kinase C beta I
•Recombinant Human Protein Kinase C delta
•Recombinant Human Protein Kinase C e

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Protein kinase C gamma mutations in spinocerebellar ataxia 14 increase kinase activity and alter membrane targeting.
Brain 2005 Feb;128(Pt 2):436-42
2. Molecular interaction model for the C1B domain of protein kinase C-gamma in the complex with its activator phorbol-12-myristate-13-acetate in water solution and lipid bilayer.
J Med Chem 2004 Dec 16;47(26):6547-55
3. Acute restraint stress reduces protein kinase C gamma in the hippocampus of C57BL/6 but not DBA/2 mice.
Neurosci Lett 2004 Sep 30;368(3):293-6
4. Mutation in the catalytic domain of protein kinase C gamma and extension of the phenotype associated with spinocerebellar ataxia type 14.
Arch Neurol 2004 Aug;61(8):1242-8
5. Spatial learning in rats is impaired by microinfusions of protein kinase C-gamma antisense oligodeoxynucleotide within the nucleus accumbens.
Neurobiol Learn Mem 2004 Mar;81(2):120-36
6. Neuronal protein kinase C gamma-dependent proliferation and hypertrophy of spinal cord astrocytes following repeated in vivo administration of morphine.
Eur J Neurosci 2004 Jan;19(2):479-84

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中華民國95年06月06日更新