Certificate of Analysis and Data Sheet

Source:
SF9 insect cells.

Catalog No.
PKA-318

Description :

Recombinant Human PKC-betaI produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-671 and having a molecular mass of 80 KD. PKC-bI is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human PKC-b I is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.

Unit Definition:

2500 units/mg. One unit of PKCβΙ activity transfers 1 nmole of phosphate to a PKC substrate peptide per minute at 30蚓. Recombinant human active PKCβΙ also phosphorylates full length myelin basic protein, histone, and α pseudosubstrate peptide. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.

Storage:

Store at 4蚓 if entire vial will be used within 2-4 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PRKCB1
Synonyms:PKCB, PRKCB

Protein synonyms/aliases:

Protein kinase C beta type (EC 2.7.1.37)
    (PKC-beta)
    (PKC-B).

Protein Family:

Belongs to the Ser/Thr protein kinase family. PKC

Protein Domains:

Domains:
•IPR002219   Protein kinase C, phorbol ester/diacylglycerol binding
•IPR000008   C2
•IPR000719   Protein kinase
•IPR000961   Protein kinase, C-terminal

Related proteins:

•Recombinant Human Protein Kinase C alpha
•Recombinant Human Protein Kinase C beta II
•Recombinant Human Protein Kinase C gamma
•Recombinant Human Protein Kinase C delta
•Recombinant Human Protein Kinase C e

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Biphasic effects of angiotensin II and receptor antagonism on aggregability and protein kinase C phosphorylation in human platelets.
Thromb Haemost 2005 Nov;94(5):1012-8
2. Protein kinase C mediates erythrocyte " programmed cell death " following glucose depletion.
Am J Physiol Cell Physiol 2006 Jan;290(1):C244-53
3. [The role of phosphoinositides, protein kinase C and protein kinase A in the K+ regulatory signal transduction in human adrenocortical cells]
Ukr Biokhim Zh 2005 Jan-Feb;77(1):65-71
4. Protein kinase C immunoreactivity in the pigmented and albino rat retina.
Eur J Neurosci 2005 Nov;22(10):2481-8
5. Expression of protein kinase C alpha in biopsies and surgical specimens of human hepatocellular carcinoma.
Chin J Physiol 2005 Sep 30;48(3):139-43
6. Apoptosis of antigen-specific T lymphocytes upon the engagement of CD8 by soluble HLA class I molecules is Fas ligand/Fas mediated: evidence for the involvement of p56lck, calcium calmodulin kinase II, and Calcium-independent protein kinase C signaling pathways and for NF-kappaB and NF-AT nuclear translocation.
J Immunol 2005 Dec 1;175(11):7244-54

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中華民國95年06月06日更新