Certificate of Analysis and Data Sheet

Source:
SF9 insect cells.

Catalog No.
PKA-218

Description :

Recombinant Human PKC-a produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-672 and having a molecular mass of 80 KD. Recombinant PKC-alpha is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human PKC-a is supplied at a of 0.3mg/ml in 50mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mMEDTA, 0.2mM PMSF and 0.03% Brij-35.

Unit Definition:

7200-9000 units/mg. One unit of PKC-alpha activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30蚓.
Recombinant human active PKC-a also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3 beta. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.

Storage:

Store at 4蚓 if entire vial will be used within 2-4 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PRKCA
Synonyms:PKCA, PRKACA

Protein synonyms/aliases:

Protein kinase C alpha type (EC 2.7.1.37)
    (PKC-alpha)
    (PKC-A).

Protein Family:

Belongs to the Ser/Thr protein kinase family. PKC

Protein Domains:

Domains:
•IPR002219   Protein kinase C, phorbol ester/diacylglycerol binding
•IPR000008   C2
•IPR000719   Protein kinase
•IPR000961   Protein kinase, C-terminal

Related proteins:

•Recombinant Human Protein Kinase C beta II
•Recombinant Human Protein Kinase C beta I
•Recombinant Human Protein Kinase C gamma
•Recombinant Human Protein Kinase C delta
•Recombinant Human Protein Kinase C e

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Expression of protein kinase C alpha in biopsies and surgical specimens of human hepatocellular carcinoma.
Chin J Physiol 2005 Sep 30;48(3):139-43
2. Oxidant-mediated activation of cytosolic phospholipase a(2) in pulmonary endothelium: role of protein kinase C alpha and a pertussis toxin-sensitive protein.
Endothelium 2005 May-Jun;12(3):121-31
3. KiSS1 suppresses metastasis in human ovarian cancer via inhibition of protein kinase C alpha.
Clin Exp Metastasis 2005;22(5):369-76
4. Protein kinase C-alpha regulation of gallbladder Na+ transport becomes progressively more dysfunctional during gallstone formation.
J Lab Clin Med 2005 Oct;146(4):227-37
5. Nuclear localization of protein kinase C-alpha induces thyroid hormone receptor-alpha1 expression in the cardiomyocyte.
Am J Physiol Heart Circ Physiol 2006 Jan;290(1):H381-9
6. Transcriptional involvement of protein kinase C-alpha isozyme in amphetamine-mediated appetite suppression.
Eur J Neurosci 2005 Aug;22(3):715-23

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中華民國95年06月06日更新