Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells

Catalog No.
PKA-207

Description :

Recombinant Human Protein Kinase B is a glycosylated polypeptide having a molecular mass of 59.1kDa.
Inactive enzyme, suitable for negative control experiments or for phosphorylation as a substrate.
Recombinant Protein Kinase B is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human PKB alpha is supplied in 50% glycerol.

Storage:

Recombinant PKB should be stored at 4蚓 if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20蚓.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

None.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Protein kinase B-alpha inhibits human pyruvate dehydrogenase kinase-4 gene induction by dexamethasone through inactivation of FOXO transcription factors.
Diabetes 2004 Apr;53(4):899-910
2. Protein kinase B alpha/Akt1 regulates placental development and fetal growth.
J Biol Chem 2003 Aug 22;278(34):32124-31
3. Mechanisms of FOXC2- and FOXD1-mediated regulation of the RI alpha subunit of cAMP-dependent protein kinase include release of transcriptional repression and activation by protein kinase B alpha and cAMP.
J Biol Chem 2002 Jun 21;277(25):22902-8
4. Fc alpha receptor cross-linking causes translocation of phosphatidylinositol-dependent protein kinase 1 and protein kinase B alpha to MHC class II peptide-loading-like compartments.
J Immunol 2001 May 1;166(9):5585-93
5. Opposing effects of protein kinase C delta and protein kinase B alpha on H(2)O(2)-induced apoptosis in CHO cells.
Biochem Biophys Res Commun 1999 Nov 2;264(3):840-6
6. Mutational analysis of the coding regions of the genes encoding protein kinase B-alpha and -beta, phosphoinositide-dependent protein kinase-1, phosphatase targeting to glycogen, protein phosphatase inhibitor-1, and glycogenin: lessons from a search for genetic variability of the insulin-stimulated glycogen synthesis pathwa y of skeletal muscle in NIDDM patients.
Diabetes 1999 Feb;48(2):403-7