Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells.

Catalog No.
PKA-333

Description :

The PI3Kb isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation of PI3Kb is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2). It was also shown that PI3Kb is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr, HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis. PI3Kb is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line. It was shown by several research groups that the catalytic subunit of PI3Kb can be activated by Gbg subunits of G-protein coupled receptors. Recombinant Human Phosphoinositide 3-kinase beta is a glycosilated protein having a molecular weight as follows: p85α chain 83.5 kDa, p110β chain 124.3 kDa.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

0.5mg/ml solution in 10 mM Hepes, pH 7.5, 100 mM NaCl, 2.5 mM MgCl2, and 50% glycerol.

Storage:

Recombinant Human PI3Kb although stable at 14⁰C for 1 week, should be stored desiccated below
-18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by SDS Page.

Biological Activity:

~ 3 nmol/mg/min using phosphatidylinositol as the substrate.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

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