Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells.

Catalog No.
PKA-332

Description :

PI3Kγ is highly expressed in cells of hematopoietic origin. It plays an important role in dendritic cell (DC) trafficking and in the activation of specific immunity. PI3Kγ-/- mice showed a reduced ability to respond to chemokines, and had a selective defect in the number of skin Langerhans cells and in lymph node CD8alpha- DCs. In macrophages, the chemokine RANTES/CCL5 activates the small GTPase Rac1 and its downstream target PAK2. This response depends on Gi activation and largely on the subsequent triggering of PI3Kγ. Distinct regions within the p101 regulatory subunit primary stucture are responsible for interaction with PI3Kγ and Gbg. The PI3Kγ binding site is confined to the N-terminus, whereas binding to Gbg is mediated by the C-terminus of p101. The PI3Kγ catalytic subunit carries a N-terminal His6 affinity Tag, whereas the p101 adaptor subunit carries a N-terminal GSTTag. The molecular weight for p110g chain is 126.3 kDa (without Tag) and the p101 chain 101 kDa (without Tag).

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

0.5mg/ml solution in 25 mM Tris, pH 8.0, 50 mM NaCl, 0.5 mM MgCl2, and 50% glycerol.

Storage:

Recombinant Human PI3Kgamma although stable at 14⁰C for 1 week, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by SDS Page.

Biological Activity:

~ 3 nmol/mg/min using phosphatidylinositol as the substrate.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

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