Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells.

Catalog No.
PKA-331

Description :

The PI3KγD946A protein is a catalytically inactive mutant of PI3Kγ with a D946A mutation in the ATP binding site. This recombinant catalytically inactive protein can be used as a negative control in any kind of PI3Kγ kinase activity studies. Recombinant full length PI3KγDA mutant (126.3 kDa without Tag) carries a N-terminal GST-Tag which facilitates the protein旧 application in typical GST pull-down assays.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

0.5mg/ml solution in 25 mM Tris, pH 8.0, 50 mM NaCl, 0.5 mM MgCl2, and 50% glycerol.

Storage:

Recombinant Human PI3Kgamma although stable at 14⁰C for 1 week, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by SDS Page.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

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