Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-304

Background:

Protein gene product-9.5(PGP9.5) ,known as ubiquitin carboxyl-terminal esterase , is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. The protein which has a molecular weight of 27kDa was first identified by high resolution two dimensional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibres at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum.

Description :

Recombinant PGP-9.5 shows a band at 36kDa on SDS-PAGE commassie blue stained gel. Recombinant PGP9.5 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered liquid.

Formulation:

100ug/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability:

Store vial at -20C^o to -80C^o. When stored at the recommended temperature, recombinant PGP9.5 is stable for 12 months.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates:

Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human PGP-9.5.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:UCHL1

Protein synonyms/aliases:

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC 3.4.19.12)
    (EC 6.-.-.-)
    (UCH-L1)
    (Ubiquitin thiolesterase L1)
    (Neuron cytoplasmicprotein 9.5)
    (PGP 9.5)
    (PGP9.5).

Protein Family:

Belongs to the peptidase C12 family.

Protein Domains:

Domains:
•IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 1

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. Protein gene product 9.5 and ubiquitin are expressed in metabolically active epithelial cells of normal and pathologic human kidney.
Nephrol Dial Transplant 2005 Dec;20(12):2714-9
2. Expression of protein gene product 9.5 (PGP9.5)/ubiquitin-C-terminal hydrolase 1 (UCHL-1) in human myeloma cells.
Br J Haematol 2004 Nov;127(3):292-8
3. Granular cell tumor: immunohistochemical assessment of inhibin-alpha, protein gene product 9.5, S100 protein, CD68, and Ki-67 proliferative index with clinical correlation.
Arch Pathol Lab Med 2004 Jul;128(7):771-5
4. Demonstration of substance P, calcitonin gene-related peptide, and protein gene product 9.5 containing nerve fibers in human cervical facet joint capsules.
Spine 2004 Jun 1;29(11):1182-6
5. Expression of protein gene product 9.5 in lepromatous eyes showing ciliary body nerve damage and a " dying back " phenomenon in the posterior ciliary nerves.
Br J Ophthalmol 2004 Feb;88(2):178-81
6. Distribution of protein gene product 9.5-immunopositive and NADPH-diaphorase-positive neurons in the common marmoset (Callithrix jacchus) accessory olfactory bulb.
J Vet Med Sci 2003 Dec;65(12):1307-11

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中華民國95年06月06日更新