Source:
E.Coli
Catalog No.
CYT-337
Human placental GH (hPGH) replaces pituitary GH during pregnancy. hPGH is correlated to serum IGF-I in normal pregnancies and in pregnancies complicated by fetoplacental disorders Description: Recombinant Placental Human GH-20K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20498 Dalton. Predicted pI=8.20. PGH-20K-pl is devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary GHs.
Placental Growth Hormone is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Placental Growth Hormone protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 11. Solubility: It is recommended to reconstitute and dilute the lyophilized Placental-GH in 0.4% NaHCO3 adjusted to pH 11 , which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Placental Growth Hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Placental Growth Hormone should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Thr-Ile. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's Placental Growth Hormone-20K is fully biologically active when compared to World Health Organization (WHO) reference standard.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Recombinant Placental Growth Hormone.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.14 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Placental Growth Hormone-20K as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:hGH-V
Placental growth hormone 20kDa isoform precursor.
Domains:
•IPR001400 Somatotropin hormone
