Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells.

Catalog No.
PRO-303

Background:

Description :

Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis.
Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity (< 10 % of SLE patients). However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE.
Recombinant Human PCNA produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 29,794 dalton.
Recombinant PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered clorless solution.

Formulation:

Recombinant Human PCNA is supplied at a of 0.9mg/ml in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% (SDS-PAGE).

Immunological functions:

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera

Applications:

Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Coating concentration:

0.5-1.1 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:PCNA

Protein synonyms/aliases:

Proliferating cell nuclear antigen (PCNA)
    (Cyclin).

Protein Family:

Belongs to the PCNA family.

Protein Domains:

Domains:
•IPR000730   Proliferating cell nuclear antigen, PCNA

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases eta and REV1.
Proc Natl Acad Sci U S A 2005 Dec 20;102(51):18361-6
2. Epidermal growth factor receptor and proliferating cell nuclear antigen expression in urine ThinPrep specimens.
Cytop athology 2005 Dec;16(6):303-8
3. Immunohistochemical localization of cytokeratin 19, involucrin and proliferating cell nuclear antigen (PCNA) in cultured human gingival epithelial sheets.
J Int Acad Periodontol 2005 Oct;7(4):129-34
4. Galectin-3 and proliferating cell nuclear antigen (PCNA) expression in papillary thyroid carcinoma.
Exp Oncol 2005 Sep;27(3):210-4
5. The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity.
J Mol Biol 2005 Nov 11;353(5):980-9
6. Phosphorylation of human DNA polymerase lambda by the cyclin-dependent kinase Cdk2/cyclin A complex is modulated by its association with proliferating cell nuclear antigen.
Nucleic Acids Res 2005 Sep 20;33(16):5354-61

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中華民國95年06月06日更新