Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-420

Background:

Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4 kDa. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dosedependent down-regulation of osteocrin expression, suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.

Description:

The recombinant Human Osteocrin is produced with N-terminal fusion of HisTag. The Human Osteocrin His-Tagged Fusion Protein is 13.6 kDa containing 106 amino acid residues of the human Osteocrin and 16 additional amino acid residues ?HisTag (bold).
MRGSHHHHHH GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL ENDVIETKKK RSFSGFGSPL DRLSAGSVDH KGKQRKVVDH PKRRFGIPMD RIGRNRLSNS RG

Physical Appearance:

Sterile Filtered lyophilized (freeze-dried) powder.

Formulation:

Sterile filtered and lyophilized from 0.5 mg/ml in 5mM TRIS, 25mM NaCl, pH 7.5.

Specificity:

The amino acid sequence of the recombinant human Osteocrin is 100% homologous to the amino acid sequence of the human Osteocrin without signal sequence.

Purification Method:

One-step procedure using affinity Ni-NTA chromatography.

Purity:

>95 % (SDS-PAGE analyzed).

Reconstitution:

Add 0.2 ml of dH2O and let the lyophilized pellet dissolve completely. Storage: Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4蚓 for a limited period of time; it does not show any change after two weeks at 4蚓.

Stability/Shelf Life:

The lyophilized protein remains stable until the expiry date when stored at -20蚓.

Applications:

Western blotting, ELISA

References:

?Thomas G, Moffatt P, Salois P, Gaumond MH, Gingras R, Godin E, Miao D, Goltzman D, Lanctot C. Osteocrin, a novel bone-specific secreted protein that modulates the osteoblast phenotype. J Biol Chem. 2003 Dec 12;278(50):50563-71.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

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Gene:

Name:OSTN

Protein synonyms/aliases:

Osteocrin precursor (Musclin).

Protein Family:

Protein Domains:

Domains:

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Characterization of osteocrin expression in human bone.
J Histochem Cytochem 2005 Oct;53(10):1181-7
2. Osteocrin, a novel bone-specific secreted protein that modulates the osteoblast phenotype.
J Biol Chem 2003 Dec 12;278(50):50563-71

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中華民國95年06月06日更新