Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ENZ-298

Background :

NSE is the γγ isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells. NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival.

Description :

Recombinant Human NSE is expressed in E. coli containing 433 amino acids 2-434 fused to an amino terminal hexahistidine tag.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant NSE is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).

Stability:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Applications:

Recombinant Human NSE can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments.
The biological activity of this product has not yet been tested.

Purity:

Greater than 95% (SDS-PAGE). Single band on Western Blot.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:ENO2

Protein synonyms/aliases:

Gamma-enolase (EC 4.2.1.11)
    (2-phospho-D-glycerate hydro-lyase)
    (Neural enolase)
    (Neuron-specific enolase)
    (NSE)
    (Enolase 2).

Protein Family:

Belongs to the enolase family.

Protein Domains:

Domains:
•IPR000941   Enolase

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. The prognostic significance of circulating neuroendocrine markers chromogranin a, pro-gastrin-releasing peptide and neuron-specific enolase in patients with advanced non-small-cell lung cancer.
Tumour Biol 2006;27(1):8-16
2. Neuron-specific enolase and lung cancer.
Am J Clin Oncol 2005 Dec;28(6):586-90
3. [Serum S-100B protein and neuron-specific enolase after traumatic brain injury]
No Shinkei Geka 2005 Nov;33(11):1073-80
4. Diagnostic utility of serum and pleural fluid carcinoembryonic antigen, neuron-specific enolase, and cytokeratin 19 fragments in patients with effusions from primary lung cancer.
Chest 2005 Oct;128(4):2298-303
5. Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice.
Clin Chem Lab Med 2005;43(11):1215-7
6. [Neuron specific enolase]
Nippon Rinsho 2005 Aug;63 Suppl 8:717-9

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中華民國95年06月06日更新