Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-274

Description :

Recombinant Human NAP-2 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 70 amino acids and having a molecular mass of 7609 Dalton. Recombinant NAP-2 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant Neutrophil-activating peptide 2, lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized NAP-2 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized NAP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution NAP-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Glu-Leu-Arg-Cys

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's NAP-2 is fully biologically active when compared to standard. The specific activity as determined by the ability of NAP-2 to chemoattract human neurotrophils using a concentration of 1-10 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of NAP-2.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of NAP-2 as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:PPBP
Synonyms:CTAP3, SCYB7, TGB1, THBGB1

Protein synonyms/aliases:

Platelet basic protein precursor (PBP)
    (Small inducible cytokine B7)
    (CXCL7)
    (Leukocyte-derived growth factor)
    (LDGF)
    (Macrophage-derivedgrowth factor)
    (MDGF)

Precursor's functional components:

Connective tissue-activating peptideIII (CTAP-III)
    (Low-affinity platelet factor IV)
    (LA-PF4)
TC-2;Connective tissue-activating peptide III(1-81)
    (CTAP-III(1-81))
Beta-thromboglobulin (Beta-TG)
Neutrophil-activating peptide 2(74)
    (NAP-2(74))
Neutrophil-activating peptide 2(73)
    (NAP-2(73))
Neutrophil-activating peptide 2 (NAP-2)
TC-1
Neutrophil-activating peptide 2(1-66)
    (NAP-2(1-66))
Neutrophil-activating peptide 2(1-63)
    (NAP-2(1-63)) .

Protein Family:

Belongs to the intercrine alpha (chemokine CxC)

Protein Domains:

Domains:
•IPR001811 Small chemokine, interleukin-8-like

Related proteins:

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. Relationship between neutrophil-binding affinity and suitability for infection imaging: comparison of (99m)Tc-labeled NAP-2 (CXCL-7) and 3 C-terminally truncated isoforms.
J Nucl Med 2004 Jul;45(7):1217-23
2. Chemotactic activity of human blood leukocytes in plasma treated with EDTA: chemoattraction of neutrophils about monocytes is mediated by the generation of NAP-2.
J Leukoc Biol 2002 Jul;72(1):175-82
3. The CXC chemokine NAP-2 mediates differential heterologous desensitization of neutrophil effector functions elicited by platelet-activating factor.
J Interferon Cytokine Res 2002 Feb;22(2):257-67
4. Thrombin-activated human platelets release two NAP-2 variants that stimulate polymorphonuclear leukocytes.
Thromb Haemost 1996 Nov;76(5):780-5
5. Limited and defined truncation at the C terminus enhances receptor binding and degranulation activity of the neutrophil-activating peptide 2 (NAP-2). Comparison of native and recombinant NAP-2 variants.
J Biol Chem 1995 Mar 17;270(11):6338-44
6. Neutrophil-activating peptides NAP-2 and IL-8 bind to the same sites on neutrophils but interact in different ways. Discrepancies in binding affinities, receptor densities, and biological effects.
J Immunol 1995 Jan 15;154(2):972-4

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中華民國95年06月06日更新