Source:
E.coli
Catalog No.
CYT-444
Midkine is expressed during embryogenesis, showing an expression pattern that suggests functions in neurogenesis, cell migration, secondary organogenetic induction, and mesoderm-epithelial interaction.
The widespread downregulation of MK in the adult human is reverted in a number of cancers, in which polypeptides are able to act as both transforming growth factors and promoters of angiogenesis.
Midkine (MK), induces chemotaxis of human neutrophils and was found to trigger mobilization of intracellular calcium of these cells.
Midkine induces histamine release from rat peritoneal mast cells with a rapid response in a dosedependent manner.
Midkine is also a potent stimulator of collagen and glycosaminoglycan synthesis.
Description: Recombinant Human Midkine is manufactured with N-terminal fusion of HisTag, having a molecular mass of 14.6 kDa protein and containing 121 amino acid residues of the human Midkine and 10 additional amino acid residues - HisTag Sequence: KKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation : Sterile filtered and lyophilized from 0.5 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2.
Specificity: The amino acid sequence of the recombinant human Midkine is 100% homologous to the amino acid sequence of the human Midkine without signal sequence.
Purification Method: Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Purity: >95% (SDS-PAGE analyzed).
Solubility: Add 0.2 ml of PBS pH 7.2 and let the lyophilized pellet dissolve completely.
Stability: Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
Reconstituted protein can be stored at 4蚓 for a limited period of time; it does not show any change after two weeks at 4蚓.
Stability:
The lyophilized protein remains stable until the expiry date when stored at -20蚓.
Applications: Western blotting, ELISA References: ?Brunet-de Carvalho N, Raulais D, Rauvala H, Souttou B, Vigny M. HB-GAM/Pleiotrophin and Midkine are differently expressed and distributed during retinoic acid-induced neural differentiation of P19 cells. Growth Factors. 2003 Sep-Dec; 21(3-4): 139-49.
?Muramatsu T, Muramatsu H, Kaneda N, Sugahara K. Recognition of glycosaminoglycans by midkine. Methods Enzymol. 2003; 363: 365-76.
?Tomizawa M, Yu L, Wada A, Tamaoki T, Kadomatsu K, Muramatsu T, Matsubara S, Watanabe K, Ebara M, Saisho H, Sakiyama S, Tagawa M. A promoter region of the midkine gene that is frequently expressed in humanhepatocellular carcinoma can activate a suicide gene as effectively as the alpha-fetoprotein promoter. Br J Cancer. 2003 Sep 15; 89(6): 1086-90.
?Shimada H, Nabeya Y, Tagawa M, Okazumi S, Matsubara H, Kadomatsu K, Muramatsu T, Ikematsu S, Sakuma S, Ochiai T. Preoperative serum midkine concentration is a prognostic marker for esophageal squamous cell carcinoma. Cancer Sci. 2003 Jul; 94(7): 628-32.
?Ikematsu S, Okamoto K, Yoshida Y, Oda M, Sugano-Nagano H, Ashida K, Kumai H, Kadomatsu K, Muramatsu H, Takashi Muramatsu, Sakuma S. High levels of urinary midkine in various cancer patients. Biochem Biophys Res Commun. 2003 Jun 27; 306(2): 329-32.
Usage: This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.
Gene:
Name:MDK
Synonyms:MK1, NEGF2
Midkine precursor (MK)
(Neurite outgrowth-promoting protein)
(Midgestation and kidney protein)
(Amphiregulin-associated protein)
(ARAP)
(Neurite outgrowth-promoting factor 2).
Belongs to the pleiotrophin family.
Domains:
•IPR000762 PTN/MK heparin-binding protein
•Recombinant Human Pleiotrophin
