Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ENZ-317

Background:

Description:

Latent recombinant human pro-collagenase (MMP-13) also called collagenase-3 truncated from
C-terminal.
Recombinant Human MMP-13 produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 27kDa.
Recombinant MMP-13 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

The protein Solution (100 ug/ml, 0.1 ml) in 0.05M Tris-HCl buffer, pH 7.5, containing 0.2M NaCl, 5mM CaCl2, 20然 ZnSO4 and 0.1% BSA.

Stability:

Recombinant MMP-13 although stable at 14⁰C for 1 week, should be stored desiccated below -18⁰C.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% as determined by SDS-PAGE.

Biological Activity:

ProSpec旧 Recombinant MMP-13 activity is determined by the cleavage of fluorogenic peptide, 100 ng of enzyme activated with APMA will digest 80% fluorogenic peptide substrate (0.1ml of 20然 solution) at 35蚓 for 30 minutes, (10ug=100 assays).

Applications:

Used as a standard for assaying MMP-13 or for screening inhibitors.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:MMP13

Protein synonyms/aliases:

Collagenase 3 precursor (EC 3.4.24.-)
    (Matrix metalloproteinase-13)
    (MMP-13).

Protein Family:

Belongs to the peptidase M10A family.

Protein Domains:

Domains:
•IPR002477   Peptidoglycan-binding domain 1
•IPR001818   Peptidase M10A and M12B, matrixin and adamalysin
•IPR000585   Hemopexin

Related proteins:

•Recombinant Human Matrix Metalloproteinase-3
•Recombinant Human Matrix Metalloproteinase-8

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Matrix metalloproteinase-13 and membrane type-1 matrix metalloproteinase in peritoneal fluid of women with endometriosis.
Gynecol Endocrinol 2005 Aug;21(2):106-10
2. Suppressed TGF-beta1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment.
Lasers Surg Med 2005 Jan;36(1):38-42
3. Altered endochondral bone development in matrix metalloproteinase 13-deficient mice.
Development 2004 Dec;131(23):5883-95
4. Cyclophilin C-associated protein is a mediator for fibronectin fragment-induced matrix metalloproteinase-13 expression.
J Biol Chem 2004 Dec 31;279(53):55334-40
5. Tretinoin reverses upregulation of matrix metalloproteinase-13 in human keloid-derived fibroblasts.
Exp Dermatol 2003;12 Suppl 2:35-42
6. Cleavage of fibromodulin in cartilage explants involves removal of the N-terminal tyrosine sulfate-rich region by proteolysis at a site that is sensitive to matrix metalloproteinase-13.
J Biol Chem 2004 Feb 20;279(8):6286-95

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中華民國95年06月06日更新