Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-322

Description :

Recombinant Human Macrophage Inflammatory Protein-4 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7813 Daltons.
Human MIP-4 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a concentrated (1mg/ml) solution containing no additives

Solubility:

It is recommended to reconstitute the lyophilized MIP-4 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized MIP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution MIP-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be
Ala-Gln-Val-Gly-Thr.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's Human Macrophage Inflammatory Protein-4 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human T lymphocytes at 1.0-10.0 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant Human MIP-4.

Protein content:

MIP-4 protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Human MIP-4 as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CCL18
Synonyms:AMAC1, DCCK1, MIP4, PARC, SCYA18

Protein synonyms/aliases:

Small inducible cytokine A18 precursor (CCL18)
    (Macrophageinflammatory protein 4)
    (MIP-4)
    (Pulmonary and activation-regulatedchemokine)
    (CC chemokine PARC)
    (Alternative macrophage activation-associated CC chemokine 1)
    (AMAC-1)
    (Dendritic cell chemokine 1)
    (DC-CK1)

Precursor's functional components:

CCL18(1-68)
CCL18(3-69)
CCL18(4-69) .

Protein Family:

Belongs to the intercrine beta (chemokine CC) family.

Protein Domains:

Domains:
•IPR001811 Small chemokine, interleukin-8-like

Related proteins:

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. A Vicious Circle of Alveolar Macrophages and Fibroblasts Perpetuates Pulmonary Fibrosis via CCL18.
Am J Respir Crit Care Med 2006 Jan 13;
2. Serum pulmonary and activation-regulated chemokine/CCL18 levels in patients with systemic sclerosis: a sensitive indicator of active pulmonary fibrosis.
Arthritis Rheum 2005 Sep;52(9):2889-96
3. Elevated plasma chemokine CCL18/PARC in beta-thalassemia.
Blood Cells Mol Dis 2005 Nov-Dec;35(3):328-31
4. CCL18-stimulated upregulation of collagen production in lung fibroblasts requires Sp1 signaling and basal Smad3 activity.
J Cell Physiol 2006 Jan;206(1):221-8
5. Involvement of CC chemokine ligand 18 (CCL18) in normal and pathological processes.
J Leukoc Biol 2005 Jul;78(1):14-26
6. Novel monoclonal antibodies detect elevated levels of the chemokine CCL18/DC-CK1 in serum and body fluids in pathological conditions.
J Leukoc Biol 2005 May;77(5):739-47

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中華民國95年06月06日更新