Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-287

Description :

Recombinant Human MIP-3b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8809 Dalton. Recombinant MIP-3 beta is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant macrophage inflammatory protein 3beta, lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized MIP-3b in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized MIP-3beta although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution MIP-3b should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Thr-Asn-Asp-Ala.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's macrophage inflammatory protein 3beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human T cells using a concentration of 10-50 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of MIP-3b.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of MIP-3beta as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CCL19
Synonyms:ELC, MIP3B, SCYA19

Protein synonyms/aliases:

Small inducible cytokine A19 precursor (CCL19)
    (Macrophageinflammatory protein 3 beta)
    (MIP-3-beta)
    (EBI1-ligand chemokine)
    (ELC)
    (Beta chemokine exodus-3)
    (CK beta-11).

Protein Family:

Belongs to the intercrine beta (chemokine CC) family.

Protein Domains:

Domains:
•IPR001811 Small chemokine, interleukin-8-like

Related proteins:

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. Inhibition of generation of cytotoxic T lymphocyte activity by a CCL19/macrophage inflammatory protein (MIP)-3beta antagonist.
J Biol Chem 2004 Sep 24;279(39):40276-82
2. Production of macrophage inflammatory protein 3alpha (MIP-3alpha) (CCL20) and MIP-3beta (CCL19) by human peripheral blood neutrophils in response to microbial pathogens.
Infect Immun 2003 Jan;71(1):524-6
3. Characterization of mouse CCX-CKR, a receptor for the lymphocyte-attracting chemokines TECK/mCCL25, SLC/mCCL21 and MIP-3beta/mCCL19: comparison to human CCX-CKR.
Eur J Immunol 2002 May;32(5):1230-41
4. Expression of CCR-7, MIP-3beta, and Th-1 chemokines in type I IFN-induced monocyte-derived dendritic cells: importance for the rapid acquisition of potent migratory and functional activities.
Blood 2001 Nov 15;98(10):3022-9
5. Neutrophils produce biologically active macrophage inflammatory protein-3alpha (MIP-3alpha)/CCL20 and MIP-3beta/CCL19.
Eur J Immunol 2001 Jul;31(7):1981-8
6. The leukotriene C(4) transporter MRP1 regulates CCL19 (MIP-3beta, ELC)-dependent mobilization of dendritic cells to lymph nodes.
Cell 2000 Nov 22;103(5):757-68

採購找生工，預算最輕鬆。
回頁首↑ | 回首頁←
中華民國95年06月06日更新