Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-333

Background :

CXCL9 is a chemoattractant for activated T cells and possess angiostatic activity. Both CXCL9 and CXCL10 have been considered as important components for the anti-tumour activities of interferon-gamma (IFNgamma) and interleukin-12 in animal models. HuMIG is induced in THP-1 cells and in peripheral blood mononuclear cells by interferon-gamma but not by interferon-alpha or by lipopolysaccharide. Analysis of mouse and human genomic DNAs suggested that the Mumig and Humig genes are true mouse-human homologues.

Description :

Recombinant Human MIG (monokine induced by gamma-interferon ) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 103 amino acids and having a molecular mass of 11700 Dalton.
Recombinant Human MIG is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized MIG in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized MIG although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution MIG should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be, Thr-Pro-Val-Val-Arg.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's MIG is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract CXCL9 transfected 293 cells using 10.0-100.0 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human MIG.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Human MIG as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CXCL9
Synonyms:MIG, SCYB9

Protein synonyms/aliases:

Small inducible cytokine B9 precursor (CXCL9)
    (Gamma interferon-induced monokine)
    (MIG).

Protein Family:

Belongs to the intercrine alpha (chemokine CxC)

Protein Domains:

Domains:
•IPR001811   Small chemokine, interleukin-8-like

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. Accumulation of plasma cells expressing CXCR3 in the synovial sublining regions of early rheumatoid arthritis in association with production of Mig/CXCL9 by synovial fibroblasts.
Clin Exp Immunol 2005 Aug;141(2):363-71
2. Accumulation of CXCR3-expressing eosinophils and increased concentration of its ligands (IP10 and Mig) in bronchoalveolar lavage fluid of patients with chronic eosinophilic pneumonia.
Int Arch Allergy Immunol 2005 Jul;137(3):229-35
3. Prediction of acute renal allograft rejection by urinary monokine induced by IFN-gamma (MIG).
J Am Soc Nephrol 2005 Jun;16(6):1849-58
4. Loss of RALT/MIG-6 expression in ERBB2-amplified breast carcinomas enhances ErbB-2 oncogenic potency and favors resistance to Herceptin.
Oncogene 2005 Jun 30;24(28):4540-8
5. Synthesis of alpha-chemokines IP-10, I-TAC, and MIG are differentially regulated in human corneal keratocytes.
Invest Ophthalmol Vis Sci 2005 May;46(5):1668-74
6. CXCR3-positive B cells found at elevated frequency in the peripheral blood of patients with MALT lymphoma are attracted by MIG and belong to the lymphoma clone.
Int J Cancer 2005 May 10;114(6):896-901

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中華民國95年06月06日更新