Certificate of Analysis and Data Sheet
Source:
E.Coli
Catalog No.
PRO-336
Background:
Myoglobin is a cytoplasmic hemoprotein, expressed solely in cardiac myocytes and oxidative skeletal muscle fibers, that reversibly binds O(2) by its heme residue, a porphyrin ring:iron ion complex. Since the initial discovery of its structure over 40 years ago, wide-ranging work by many investigators has added importantly to our understanding of its function and regulation. Functionally, myoglobin is well accepted as an O(2)-storage protein in muscle, capable of releasing O(2) during periods of hypoxia or anoxia. Myoglobin is also thought to buffer intracellular O(2) concentration when muscle activity increases and to facilitate intracellular O(2) diffusion by providing a parallel path that augments simple diffusion of dissolved O(2). The use of gene targeting and other molecular biological techniques has revealed important new insights into the developmental and environmental regulation of myoglobin and provided additional functions for this hemoprotein such as scavenging nitric oxide and reactive O(2) species.
Description :
Recombinant Human MB produced in E.Coli is a non-glycosylated polypeptide chain having a molecular mass of 11.67kDa.
Physical Appearance:
Sterile Filtered brownish solution.
Formulation:
Recombinant Human Myoglobin, at a concentration of 1 mg/ml, contains phosphate-buffered saline (pH 7.4) and 0.05% NaN3.
Stability:
Recombinant Myo heme free although stable at 15
0C for 2 weeks, should be stored at 4
0C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Dimers and aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
<
Endotoxin:
Less than 0.1 ng/ug (IEU/ug) of Recombinant Myoglobin.
Usage:
This material is offered by ProSpec-TechnoGene for research,
laboratory or further evaluation purposes.
Gene:
Name:MB
Protein synonyms/aliases:
Myoglobin.
Protein Family:
Belongs to the globin family.
Protein Domains:
Domains:
•IPR000971 Globin
Related proteins:
•Recombinant Human Myoglobin Heme free
Protein links:
