Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-299

Background:

MART-1 (also known as Melan-A) is a melanocyte differentiation antigen. It is present in melanocytes of normal skin and retina, nevi and in more than 85% of melanomas. This antibody is very useful in establishing the diagnosis of metastatic melanomas and has also been shown to be positive in adrenal cortical cells and tumors derived from them making it very helpful in distinguishing adrenal cortical neoplasms from other tumors which are negative for this marker.

Description :

MART-1 or MELAN-A is newly identified melanocyte differentiation antigen recognized by autologous T lymphocytes. Six other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, Tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

purified human MART-1 protein at 100ug/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.

Stability:

Store vial at -20⁰C to -80⁰C. When stored at the recommended temperature, this protein is stable for 12 months.
Please avoid freeze-thaw cycles.

Application and Suggested Dilutions:

?ELISA
?Inhibition Assays
?Western Blotting

Characterization:

On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 18kDa.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Warning: Invalid argument supplied for foreach() in /home/prospec/public_html/cart/Smarty-2.5.0/plugins/function.interpro.php on line 30

Gene:

Name:MLANA
Synonyms:MART1

Protein synonyms/aliases:

Melanoma antigen recognized by T-cells 1 (MART-1)
    (Melan-A protein)
    (Antigen SK29-AA)
    (Antigen LB39-AA).

Protein Family:

Protein Domains:

Domains:

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Culture of melanoma cells in 3-dimensional architectures results in impaired immunorecognition by cytotoxic T lymphocytes specific for Melan-A/MART-1 tumor-associated antigen.
Ann Surg 2005 Dec;242(6):851-7, discussion 858
2. Adoptive transfer of tumor-reactive Melan-A-specific CTL clones in melanoma patients is followed by increased frequencies of additional Melan-A-specific T cells.
J Immunol 2005 Oct 1;175(7):4797-805
3. HMB-45 may be a more sensitive maker than S-100 or Melan-A for immunohistochemical diagnosis of primary oral and nasal mucosal melanomas.
J Oral Pathol Med 2005 Oct;34(9):540-5
4. Melan-A/MART-1 positive " pseudonests " in lichenoid inflammatory lesions: an uncommon phenomenon.
Am J Dermatopathol 2005 Aug;27(4):370-1
5. Re: Questionable utility of Melan-A/Mart-1 immunoperoxidase staining while doing Mohs surgery for melanoma.
Dermatol Surg 2005 Apr;31(4):495
6. Selective responsiveness to common gamma chain cytokines in peripheral blood-derived cytotoxic T lymphocytes induced by Melan-A/MART-1(27-35)targeted active specific immunotherapy.
Int J Cancer 2005 Jun 10;115(2):248-55

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新