Certificate of Analysis and Data Sheet
Source:
E.Coli
Catalog No.
CHM-314
Description :
Recombinant Human Lymphotactin produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 92 amino acids and having a molecular mass of 10007 Dalton.
Human Lymphotactin is purified by proprietary chromatographic techniques.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Human Lymphotactin was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Solubility:
It is recommended to reconstitute the lyophilized Human Lymphotactin in sterile 18MO-cm
H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Human Lymphotactin although stable at room temperature for 3 weeks, should be stored desiccated
below -18 C. Upon reconstitution Human Lymphotactin should be stored at 4 C between 2-7 days and for future use
below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity:
Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Amino-Acid Sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Ser-Glu-Val-Ser.
Dimers and aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity:
ProSpec's Lymphotactin is fully biologically active when compared to standard.
The Biological activity is calculated by its ability to chemoattract human T cells at
10.0-100.0 ng/ml.
Endotoxin:
Less than 0.1 ng/ug (IEU/ug) of Lymphotactin.
Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of Lymphotactin as a Reference Standard.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:XCL1
Synonyms:LTN, SCYC1
Protein synonyms/aliases:
Lymphotactin precursor (XCL1)
(Cytokine SCM-1)
(ATAC)
(Lymphotaxin)
(SCM-1-alpha)
(Small inducible cytokine C1)
(XC chemokine ligand 1).
Protein Family:
Belongs to the intercrine gamma family.
Protein Domains:
Domains:
•IPR001811 Small chemokine, interleukin-8-like
Protein links: