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Recombinant Human Lymphotactin

Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-314

Description :

Recombinant Human Lymphotactin produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 92 amino acids and having a molecular mass of 10007 Dalton.
Human Lymphotactin is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Human Lymphotactin was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized Human Lymphotactin in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Human Lymphotactin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Human Lymphotactin should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Ser-Glu-Val-Ser.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's Lymphotactin is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human T cells at 10.0-100.0 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Lymphotactin.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Lymphotactin as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


Gene:

Name:XCL1
Synonyms:LTN, SCYC1

Protein synonyms/aliases:

Lymphotactin precursor (XCL1)
    (Cytokine SCM-1)
    (ATAC)
    (Lymphotaxin)
    (SCM-1-alpha)
    (Small inducible cytokine C1)
    (XC chemokine ligand 1).

Protein Family:

Belongs to the intercrine gamma family.

Protein Domains:


Domains:
IPR001811   Small chemokine, interleukin-8-like

Protein links:

Recombinant Human Lymphotactin protein domain

Recombinant Human Lymphotactin protein family


Precursor- Protein structure and amino acid sequence:


Latest Publications:

1. Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity.
J Immunol Methods 2005 Jul;302(1-2 ):136-44
2. Combined CD4+ Th1 effect and lymphotactin transgene expression enhance CD8+ Tc1 tumor localization and therapy.
Gene Ther 2005 Jun;12(12):999-1010
3. Epigenetic regulation of protein phosphatase 2A (PP2A), lymphotactin (XCL1) and estrogen receptor alpha (ER) expression in human breast cancer cells.
Cancer Biol Ther 2004 Dec;3(12):1304-12
4. Lymphotactin enhances the in-vitro immune efficacy of dendritoma formed by dendritic cells and mouse hepatocellular carcinoma cells.
J Zhejiang Univ Sci 2004 Oct;5(10):1255-61
5. Synergistic effect of lymphotactin and interferon gamma-inducible protein-10 transgene expression in T-cell localization and adoptive T-cell therapy of tumors.
Int J Cancer 2004 May 10;109(6):817-25
6. Induction of C chemokine XCL1 (lymphotactin/single C motif-1 alpha/activation-induced, T cell-derived and chemokine-related cytokine) expression by HIV-1 Tat protein.
J Immunol 2004 Feb 1;172(3):1888-95

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中華民國95年06月06日更新