Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CYT-281

Background:

Leukaemia inhibitory factor (LIF) is a multifunctional cytokine, which plays a role in growth-promotion and differentiation, regulates calcium and bone metabolism, induces acute phase proteins and causes cachexia in organisms with neoplastic disorders. Moreover, LIF participates in the induction of inflammation, and therefore represents an important pathogenic factor of many disorders. A member of the gp130 cytokine family, is involved in osteoarticular tissue metabolism and might participate in osteogenesis. Immunohistochemical staining showed that LIF is expressed in hypertrophic chondrocytes and vascular sprouts of cartilage and bone during rat and human osteogenesis.

Description :

Recombinant Human LIF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19700 Dalton. Recombinant LIF purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile liquid clear solution

Formulation:

The sterile protein solution (0.2mg/ml) contains 100mM phosphate buffer pH-7.2

Stability:

rHuLIF although stable at 15蚓 for 3 weeks, should be stored at 4蚓. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates:

Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human LIF.

Endotoxin:

The ED50 was determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of > 1 x 10⁸ units/mg.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:LIF
Synonyms:HILDA

Protein synonyms/aliases:

Leukemia inhibitory factor precursor (LIF)
    (Differentiation-stimulating factor)
    (D factor)
    (Melanoma-derived LPL inhibitor)
    (MLPLI)
    (Emfilermin).

Protein Family:

Belongs to the LIF/OSM family.

Protein Domains:

Domains:
•IPR001581   LIF/OSM

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Endoplasmic reticulum aminopeptidase1 mediates leukemia inhibitory factor-induced cell surface HLA-G expression in JEG-3 choriocarcinoma cells.
Endocrinology 2006 Jan 26;
2. Cross-talk between bone morphogenetic protein 2 and leukemia inhibitory factor through ERK 1/2 and Smad1 in protection against doxorubicin-induced injury of cardiomyocytes.
J Mol Cell Cardiol 2006 Feb;40(2):224-233
3. Developmental exposure to corticosterone: behavioral changes and differential effects on leukemia inhibitory factor (LIF) and corticotropin-releasing hormone (CRH) gene expression in the mouse.
Psychopharmacology (Berl) 2006 Jan 14;:1-8
4. [The re lationship between leukemia inhibitory factor and neurokinin receptors in a rat model of asthma.]
Zhonghua Jie He He Hu Xi Za Zhi 2005 Dec;28(12):820-4
5. Leukemia inhibitory factor induces endothelial differentiation in cardiac stem cells.
J Biol Chem 2005 Dec 28;
6. Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation.
Acta Pharmacol Sin 2006 Jan;27(1):80-90

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中華民國95年06月06日更新