Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-327

Background:

LA/SS-B (SS-B Antigen) is a cytoplasmic or nuclear ribonucleoproteins carrying LA determinant.SS-B/La is a nuclear protein of 48 kilodaltons with two structural domains of Mr 28,000 and Mr 23,000 generated by proteolytic cleavage.The human La (SS-B) autoantigen is an abundantly expressed putative RNA chaperone, functioning in various intracellular processes involving RNA.

Description :

Recombinant Human LA/SS-B produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 47.6kDa. Recombinant LA/SS-B is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

Recombinant Human LA/SS-B ,at a concentration of 1.6mg/ml contains 10mM Sodium Phosphate, 150mM NaCl and 0.1% NaN3 (pH: 7.2).

Stability:

Recombinant LA/SS-B although stable at 4^oC for 3 weeks, should be stored desiccated below -18 C.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of LA/SS-B

Protein content:

Protein quantitation was carried out by using:
0.25 - 2.0 mg/ml Bradford assay vs. BSA

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:SSB

Protein synonyms/aliases:

Lupus La protein (Sjogren syndrome type B antigen)
    (SS-B)
    (Laribonucleoprotein)
    (La autoantigen).

Protein Family:

Protein Domains:

Domains:
•IPR006630   RNA-binding protein Lupus La
•IPR000504   RNA-binding region RNP-1 (RNA recognition motif)

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Neo-epitopes are required for immunogenicity of the La/SS-B nuclear antigen in the context of late apoptotic cells.
Clin Exp Immunol 2006 Feb;143(2):237-48
2. Expression of Ro/SS-A and La/SS-B determined by immunohistochemistry in healthy, inflamed and autoimmune diseased human tissues: a generalized phenomenon.
Clin Exp Rheumatol 2004 May-Jun;22(3):285-92
3. The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase .
RNA 2002 Nov;8(11):1428-43
4. Antibodies to Ro/SS-A and La/SS-B in systemic lupus erythematosus and other autoimmune disorders.
J Assoc Physicians India 2002 Jun;50:762-5
5. Restricted specificity of intermolecular spreading to endogenous La (SS-B) and 60 kDa Ro (SS-A) in experimental autoimmunity.
Scand J Immunol 2002 Aug;56(2):168-73
6. Detailed analysis of the phosphorylation of the human La (SS-B) autoantigen. (De)phosphorylation does not affect its subcellular distribution.
Biochemistry 2000 Mar 21;39(11):3023-33

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中華民國95年06月06日更新