Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells.

Catalog No.
PRO-397

Background:

Ku antigen consists of two polypeptides (70 kDa and 80 kDa; the p80 subunit is occasionally called p86). The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V(D)J recombination, telomere maintenance, and the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome (up to 20% of patients) but have also been detectable in about 20 % of patients with primary pulmonary hypertension (as a complication of overlap), in 5-10 % of SLE and MCTD patients, and in up to 20 % of patients with primary Sj鐷ren's syndrome. In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent.

Description :

Recombinant Human KuP70/P80 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 43,591 dalton. Recombinant Ku(P70/P80) is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered clorless solution.

Formulation:

Recombinant Human KuP70/P80 is supplied at a of 0.9mg/ml in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% (SDS-PAGE).

Immunological functions:

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Applications:

Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera.

Coating concentration:

0.2-0.6 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals

Latest Publications:

1. Ku protein as a potential human T-cell leukemia virus type 1 (HTLV-1) Tax target in clastogenic chromosomal instability of mammalian cells.
Retrovirology 2005 Jul 13;2(1):45
2. Ku protein targeting by Ku70 small interfering RNA enhances human cancer cell response to topoisomerase II inhibitor and gamma radiation.
Mol Cancer Ther 2005 Apr;4(4):529-36
3. The Ku protein complex is involved in length regulation of Drosophila telomeres.
Genetics 2005 May;170(1):221-35
4. The double life of the Ku protein: facing the DNA breaks and the extracellular environment.
Cell Cycle 2005 Mar;4(3):438-41
5. Association between autoantibodies to the Ku protein and DPB1*.
Arthritis Rheum 2005 Feb;52(2):668-9
6. The Ku protein complex interacts with YY1, is up-regulated in human heart failure, and represses alpha myosin heavy-chain gene expression.
Mol Cell Biol 2004 Oct;24(19):8705-15