Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CYT-270

Description :

Recombinant Human Insulin produced in E.Coli is a two chain, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton.
Recombinant Insulin is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant Human Insulin was lyophilized from a concentrated (1mg/ml) solution with no additives.

Solubility:

It is recommended to reconstitute the lyophilized recombinant Insulin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Recombinant Human Insulin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Insulin should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's recombinant Insulin is fully biologically active when compared to World Health Organization (WHO) reference standard which is 28 units/mg.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of recombinant Insulin.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of Insulin as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:INS

Protein synonyms/aliases:

Insulin precursor

Precursor's functional components:

Insulin B chain
Insulin A chain .

Protein Family:

Belongs to the insulin family.

Protein Domains:

Domains:
•IPR004825   Insulin/IGF/relaxin

Related proteins:

•Recombinant Human Insulin Like Growth Factor-I (IGF-I)
•Recombinant Chicken Insulin Like Growth Factor-I
•Recombinant Murine Insulin Like Growth Factor-I
•Recombinant Salmon/Trout Insulin Like Growth Factor-I
•Recombinant Rat Insulin Like Growth Factor-I
•Recombinant Chicken Insulin Like Growth Factor-II
•Recombinant Human Insulin Like Growth Factor-II

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Systemic allergy to human insulin and its rapid and long acting analogs: successful treatment by continuous subcutaneous insulin lispro infusion.
Diabetes Metab 2005 Sep;31(4 Pt 1):391-400
2. Overexpression for commercial production of recombinant human insulin as A-chain and B-chain fusion protein in Escherichia coli through genetically engineered plasmids.
Indian J Pathol Microbiol 2004 Oct;47(4):569-73
3. Pancreas transplantation for treatment of generalized allergy to human insulin in type 1 diabetes.
Transplant Proc 2005 Jul-Aug;37(6):2839
4. Short-acting insulin analogues vs. human insulin for diabetes.
Am Fam Physician 2005 Sep 1;72(5):805-7
5. [Outcome of non-pharmacologic treatment in a gestational diabetic woman with high insulin resistance HOMA-IR index and allergy to human insulin. Case report]
Ginekol Pol 2005 May;76(5):403-8
6. Glucagon-like peptide-1 stimulates human insulin promoter activity in part through cAMP-responsive elements that lie upstream and downstream of the transcription start site.
J Endocrinol 2005 Aug;186(2):353-65

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中華民國95年06月06日更新